Method for integrating biological tissues with a vascular system

Abstract

The present invention provides a method of constituting a tissue construct in vitro using a tissue without depending on scaffold materials. A method of integrating a biological tissue with a vascular system in vitro, comprising coculturing a biological tissue with vascular cells and mesenchymal cells. A biological tissue which has been integrated with a vascular system by the above-described method. A method of preparing a tissue or an organ, comprising transplanting the biological tissue described above into a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. A method of regeneration or function recovery of a tissue or an organ, comprising transplanting the biological tissue described above into a human or a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. A method of preparing a non-human chimeric animal, comprising transplanting the biological tissue described above into a non-human animal and differentiating the biological tissue into a tissue or organ in which vascular networks have been constructed. A method of evaluating a drug, comprising using at least one member selected from the group consisting of the biological tissue described above, the tissue or organ prepared by the method described above, and the non-human chimeric animal prepared by the method described above. A composition for regenerative medicine, comprising a biological tissue which has been integrated with a vascular system by the method described above.

1 . A method of integrating a biological tissue with a vascular system in vitro, comprising coculturing a biological tissue with vascular cells and mesenchymal cells. 
     
     
         2 . The method of  claim 1 , wherein the biological tissue is cocultured with vascular cells and mesenchymal cells without using scaffold materials. 
     
     
         3 . The method of  claim 1  or  2 , wherein by coculturing the biological tissue with vascular cells and mesenchymal cells, the biological tissue is integrated with a vascular system so that the function of the biological tissue is maintained and/or improved. 
     
     
         4 . A biological tissue which has been integrated with a vascular system by the method of  claim 1 . 
     
     
         5 . A method of preparing a tissue or an organ, comprising transplanting the biological tissue of  claim 4  into a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. 
     
     
         6 . A method of regeneration or function recovery of a tissue or an organ, comprising transplanting the biological tissue of  claim 4  into a human or a non-human animal and differentiating the biological tissue into a tissue or an organ in which vascular networks have been constructed. 
     
     
         7 . A method of preparing a non-human chimeric animal, comprising transplanting the biological tissue of  claim 4  into a non-human animal and differentiating the biological tissue into a tissue or organ in which vascular networks have been constructed. 
     
     
         8 . A method of evaluating a drug, comprising using at least one member selected from the group consisting of the biological tissue of  claim 4 , the tissue or organ prepared by the method of  claim 5 , and the non-human chimeric animal prepared by the method of  claim 7 . 
     
     
         9 . A composition for regenerative medicine, comprising the biological tissue of  claim 4 . 
     
     
         10 . The composition of  claim 9 , which is used for preparing a tissue or an organ. 
     
     
         11 . The composition of  claim 9 , which is used for regeneration or function recovery of a tissue or an organ. 
     
     
         12 . The composition of any one of  claims 9  to  11 , wherein the biological tissue differentiates into a tissue or an organ with vascular networks upon transplantation into a living body.