Method for inducing pluripotent stem cells and pluripotent stem cells prepared by said method

Abstract

The present disclosure relates to a method for inducing pluripotent stem cells by inducing reprogramming and/or dedifferentiation of differentiated adult cells using shikimic acid, a plant extract or plant stem cells containing shikimic acid and an extract of dedifferentiated stem cells (callus), pluripotent stem cells prepared by the method and a composition containing the pluripotent stem cells. In accordance with the present disclosure, ethical concerns implicated with the use of eggs to prepare pluripotent stem cells such as embryonic stem cell can be resolved. And, because the plant stem cell extract unharmful to human is used, pluripotent stem cells with proven safety can be prepared and they may be used to develop immunocompatible cell therapy agents suited for individuals. In addition, by pluripotent stem cells from individuals having diseases, the present disclosure will be very useful in studying the cause of diseases and devolving therapeutic strategy.

1 . A method for preparing stem cells, comprising treating adult-derived cells with shikimic acid, a plant extract comprising shikimic acid, a plant stem cell extract comprising shikimic acid, or a composition comprising one or more of them. 
     
     
         2 . The method according to  claim 1 , wherein the plant stem cell extract is an extract of dedifferentiated pluripotent plant stem cells. 
     
     
         3 . The method according to  claim 1 , which comprises injecting the shikimic acid, the extract or the composition into the adult-derived cells; and culturing the cells into which the shikimic acid, the extract or the composition has been injected. 
     
     
         4 . The method according to  claim 1 , wherein the plant extract comprising shikimic acid further comprises an extract of one or more selected from a group consisting of  sequoia  ( Sequoiadendron giganteum ),  Iris pseudoacorus, Helianthus tuberosus, Picea pungens, Picea glauca, Eucalyptus sieberiana, Eucalyptus regnans, Thuja plicata, Phoenix dactylifera, Dahlia variabilis, Malus baccata, Pyrus communis, Triticum, Pinus densifloraa, Pinus thunbergii, Illicium anisatum, Magnolia grandiflora, Houttuynia cordata, Saxifraga stolonifera, Terminalia arjuna, Pistacia lentiscus, Ribes aureum, Symphytum officinalis, Actaea pachypoda, Alangium salvifollium, Gingko biloba, Veratrum viride, Dipsacus laciniatus, Agastache urticifolia, Inula helenium, Hypericum  spp.,  Commelina bengalensis, Gymnema sylvestris, Terminalia chebula, Illicium floridanum, Illicium diffengri, Illicium henryi, Illicium verum, Illicium lancealatum, Illicium pachyphyllum, Illicium anisatum, Illicium religiosum, Hemidesmus indicus, Cistus incanus, Sida acuta, Celastrus paniculata, Glycosmis muricata, Tanacetum parthenium, Triticum aestivum, Hypericum dolabriforme, Dipsacus pilosus, Triadenum walteri, Hypericum flondosum  and  Terminalia pallid.    
     
     
         5 . The method according to  claim 1 , wherein the plant stem cell extract is a callus extract. 
     
     
         6 . The method according to  claim 1 , wherein the plant stem cells are stem cells of  sequoia  and the  sequoia  is giant  sequoia  ( Sequoiadendron giganteum ). 
     
     
         7 . The method according to  claim 1 , wherein the composition comprises the shikimic acid at a concentration of 10 μM to 30 mM or comprises the extract at a concentration of 0.001 μg/mL to 2 mg/mL, based on the total volume of the composition. 
     
     
         8 . The method according to  claim 3 , which further comprises, before the injection of the shikimic acid, the extract or the composition, treating the adult-derived cells with a cell membrane permeabilizing agent. 
     
     
         9 . The method according to  claim 8 , wherein the cell membrane permeabilizing agent is streptolysin O or digitonin. 
     
     
         10 . The method according to  claim 3 , which further comprises culturing the adult-derived cells into which the shikimic acid, the extract or the composition has been injected after transferring to a feeder cell layer. 
     
     
         11 . The method according to  claim 10 , wherein the feeder cells are STO cells. 
     
     
         12 . stem cells induced from adult-derived cells by shikimic acid, a plant extract comprising shikimic acid, a plant stem cell extract comprising shikimic or a composition containing one or more of them. 
     
     
         13 . The induced pluripotent stem cells according to  claim 12 , which are prepared by the method according to  claim 1 . 
     
     
         14 . The induced pluripotent stem cells according to  claim 12 , wherein the adult-derived cells are derived from an individual to which the induced pluripotent stem cells will be administered and the stem cells are specific for the individual. 
     
     
         15 . (canceled) 
     
     
         16 . A method for cell therapy, activating stem cells, proliferating skin cells, regenerating skin or anti-aging, comprising a step of administering shikimic acid, a plant extract comprising shikimic acid, a plant stem cell extract comprising shikimic or a composition containing one or more of them to an individual in need of cell therapy, activation of stem cells, proliferation of skin cells, skin regeneration or anti-aging. 
     
     
         17 . The method according to  claim 16 , wherein the composition comprises the shikimic acid at a concentration of 10 μM to 30 mM or comprises the plant extract or the plant stem cell extract at a concentration of 0.001 μg/mL to 2 mg/mL, based on the total volume of the composition. 
     
     
         18 . The method according to  claim 16 , wherein the composition is a pharmaceutical composition or a cosmetic composition. 
     
     
         19 . The method according to  claim 16 , wherein the plant extract comprising shikimic acid is the plant extract described in  claim 4 .