US2016222402A1PendingUtilityA1

Regulatory sequences for expressing gene products in plant reproductive tissue

64
Assignee: SYNGENTA PARTICIPATIONS AGPriority: Apr 20, 2004Filed: Dec 10, 2013Published: Aug 4, 2016
Est. expiryApr 20, 2024(expired)· nominal 20-yr term from priority
Inventors:Michael Lee NuccioLawrence M. LagriminiMoez Meghji
C12N 15/827C12N 15/8271
64
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Claims

Abstract

Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants.

Claims

exact text as granted — not AI-modified
1 . A method of increasing abiotic stress tolerance in a plant, the method comprising:
 a) inserting an expression cassette into a plant cell, wherein the expression cassette comprises a trehalose-6-phosphate phosphatase polynucleotide operably linked to a 5′-regulatory polynucleotide of a MADS6 gene from  Oryza sativa  wherein the 5′-regulatory polynucleotide confers expression of the trehalose-6-phosphate phosphatase polynucleotide in maternal plant reproductive tissue;   b) regenerating transgenic plants from the plant cell of a); and   c) evaluating the plants of b) for increased abiotic stress tolerance.   
     
     
         2 . The method of  claim 1 , wherein the trehalose-6-phosphate phosphatase polynucleotide is obtained from  Oryza sativa  or maize. 
     
     
         3 . The method of  claim 2 , wherein the trehalose-6-phosphate phosphatase polynucleotide is obtained from  Oryza sativa.    
     
     
         4 . The method of  claim 1 , wherein the trehalose-6-phosphate phosphatase polynucleotide encodes a polypeptide having 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO: 532. 
     
     
         5 . The method of  claim 1 , wherein the trehalose-6-phospate phosphatase polynucleotide comprises a polynucleotide having 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO: 531. 
     
     
         6 . The method of  claim 5 , wherein the trehalose-6-phospate phosphatase polynucleotide comprises SEQ ID NO: 531. 
     
     
         7 . The method of  claim 1 , wherein the 5′-regulatory polynucleotide comprises a polynucleotide having 90%, 95%, 98%, 99% or 100% sequence identity to base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         8 . The method of  claim 7 , wherein the 5′-regulatory polynucleotide comprises base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         9 . The method of  claim 1 , wherein the 5′-regulatory polynucleotide comprises SEQ ID NO: 12. 
     
     
         10 . The method of  claim 1 , wherein the expression cassette comprises SEQ ID NO: 531 and SEQ ID NO: 12. 
     
     
         11 . The method of  claim 1 , wherein the plant is a monocot. 
     
     
         12 . The method of  claim 1 , wherein the plant is a dicot. 
     
     
         13 . The method of  claim 1 , wherein the plant is selected from the group consisting of sugarcane, sugarbeet,  sorghum,  wheat, rice, oat, barley and maize. 
     
     
         14 . The method of  claim 1 , wherein increased abiotic stress has a positive effect on grain yield relative to control plants not comprising said expression cassette. 
     
     
         15 . An expression cassette comprising a trehalose-6-phosphate phosphatase polynucleotide operably linked to a 5′-regulatory polynucleotide of a MADS6 gene from  Oryza sativa  resulting in the expression of the trehalose-6-phosphate phosphatase polynucleotide in maternal plant reproductive tissue. 
     
     
         16 . The expression cassette of  claim 15 , wherein the trehalose-6-phosphate phosphatase polynucleotide is obtained from  Oryza sativa  or maize. 
     
     
         17 . The expression cassette of  claim 16 , wherein the trehalose-6-phosphate phosphatase polynucleotide is obtained from  Oryza sativa.    
     
     
         18 . The expression cassette of  claim 15 , wherein the trehalose-6-phosphate phosphatase polynucleotide encodes a polypeptide having 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO: 532. 
     
     
         19 . The expression cassette of  claim 15 , wherein the trehalose-6-phospate phosphatase polynucleotide comprises a polynucleotide having 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO: 531. 
     
     
         20 . The expression cassette of  claim 19 , wherein the trehalose-6-phospate phosphatase polynucleotide comprises SEQ ID NO: 531. 
     
     
         21 . The expression cassette of  claim 15 , wherein the 5′-regulatory polynucleotide comprises a polynucleotide having 90%, 95%, 98%, 99% or 100% sequence identity to base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         22 . The expression cassette of  claim 21 , wherein the 5′-regulatory polynucleotide comprises base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         23 . The expression cassette of  claim 15 , wherein the 5′-regulatory polynucleotide comprises SEQ ID NO: 12. 
     
     
         24 . The expression cassette of  claim 15 , wherein the expression cassette comprises SEQ ID NO: 531 and SEQ ID NO: 12. 
     
     
         25 . A host cell comprising the expression cassette of  claim 15 . 
     
     
         26 . The host cell of  claim 25 , wherein the host cell is a plant cell. 
     
     
         27 . A transgenic plant comprising the expression cassette of  claim 15 . 
     
     
         28 . A transgenic plant comprising the expression cassette of  claim 24 . 
     
     
         29 . A transgenic plant generated by the method of  claim 1 . 
     
     
         30 . An expression cassette comprising a 5′-regulatory polynucleotide from an  Oryza sativa  OsMADS6 gene; wherein the 5′-regulatory sequence confers expression of a polynucleotide in maternal plant reproductive tissue. 
     
     
         31 . The expression cassette of  claim 30 , wherein the 5′-regulatory polynucleotide comprises a polynucleotide having 90%, 95%, 98%, 99% or 100% sequence identity to base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         32 . The expression cassette of  claim 31 , wherein the 5′-regulatory sequence comprises base pairs 13 to 1478 of SEQ ID NO: 12. 
     
     
         33 . The expression cassette of  claim 30 , wherein the 5′-regulatory polynucleotide comprises SEQ ID NO: 12. 
     
     
         34 . A host cell comprising the expression cassette of  claim 30 . 
     
     
         35 . The host cell of  claim 34 , wherein the host cell is a plant cell. 
     
     
         36 . A transgenic plant comprising the expression cassette of  claim 30 . 
     
     
         37 . The plant of  claim 36 , wherein the plant is maize.

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