US2016228437A1PendingUtilityA1

Mixed lineage kinase inhibitors and method of treatments

32
Assignee: CALIFIA BIO INCPriority: Mar 7, 2013Filed: Apr 20, 2016Published: Aug 11, 2016
Est. expiryMar 7, 2033(~6.6 yrs left)· nominal 20-yr term from priority
A61P 35/00A61P 9/04A61P 9/00A61P 9/12A61P 7/10A61P 43/00A61P 3/06A61P 3/10A61P 9/10A61P 3/04A61P 27/02A61P 29/00A61P 25/28A61P 25/16A61P 31/18A61K 31/5025A61K 31/536A61K 31/513A61P 1/16A61K 45/06A61P 13/12C07D 513/04A61K 31/427A61P 19/06A61P 15/00A61P 1/18C07D 487/04C07D 519/00A61K 31/4402A61P 25/00A61P 1/04Y02A50/30
32
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Claims

Abstract

Provided herein are imidazopyridine compounds having an inhibitory effect on mixed lineage kinases (MLKs), methods of their synthesis, and methods of their therapeutic. Also provided are pharmaceutical compositions comprising the compounds and methods of using the compounds and pharmaceutical compositions.

Claims

exact text as granted — not AI-modified
We claim: 
     
         1 . A method for treating a MLK-mediated disease comprising administering to a subject a therapeutically effective amount of a pharmaceutical composition comprising a compound having the structure of Formula I 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable isomer, isotope, enantiomer, salt, ester, prodrug, hydrate or solvate thereof, wherein 
         J has a structure of 
       
       
         
           
           
               
               
           
         
       
       where J is optionally substituted with up to four R 10 , each R 10  is independently selected from the group consisting of halo, alkyl, haloalkyl, alkoxy, haloalkoxy, —OH, and —OCOR 6 ;
 X is NR 12  or S; 
 each X 1 , X 2 , X 3 , and X 4  is independently CH or N and wherein no more than one of X 1 , X 2 , X 3 , and X 4  is N; 
 Y is —W—(CH 2 ) n —R 1 , 
 
       
         
           
           
               
               
           
         
         W is null, phenylene, or —NR 6 -phenylene, where the NR 6  is attached to the imidazopyridazine core structure of Formula I; 
         R 1  is —NR 2 R 3 , or piperazinyl, where the nitrogen atom of the piperazinyl is optionally substituted with alkyl or alkoxy; 
         R 2  is H or alkyl; 
         R 3  is selected from the group consisting of C 2 -C 10  alkyl, aryl, heteroaryl, cycloalkyl, and heterocycloalkyl, where any atom of R 3  is optionally substituted with one or more R 7 ; or R 2  and R 3  taken together with the N atom to which they are attached form a 3- to 7-membered heterocyclic ring optionally substituted with R 8 ; 
         R 4  is H or alkyl; 
         R 5  is H, alkyl, or NHR 9 ; 
         R 6  is H or alkyl; 
         each R 7  is independently alkyl, cycloalkyl, alkoxy, cycloalkoxy, cycloalkylalkyoxy, perhaloalkoxy, halo, oxo, —OH, hydroxyalkyl, —COOR 11 , or —O—(CH 2 ) m —OH; 
         R 8  is alkoxy, hydroxyalkyl, or COOR 11 ; 
         R 9  is H, alkyl, or cycloalkyl; 
         R 11  is H, or alkyl; 
         R 12  is H, or alkyl; 
         n is 0 or 1; 
         m is 1, 2, or 3; and 
         p is 1, 2, or 3; 
         with the proviso that if J is an unsubstituted benzothiophene, then R 3  is either aryl or heteroaryl, where any atom of R 3  is optionally substituted with one or more R 7 ; 
         and together with at least one pharmaceutically acceptable carrier, diluent or excipient. 
       
     
     
         2 . The method of  claim 1  wherein J is selected from the group consisting of 
       
         
           
           
               
               
           
         
       
     
     
         3 . The method of  claim 1  wherein J is 
       
         
           
           
               
               
           
         
         wherein each R 10  is independently selected from the group consisting of F, Cl, —OH, methyl, methoxy, ethoxy, propoxy, isopropoxy, and —OCOCH 3 ; and k is 0, 1, 2, or 3. 
       
     
     
         4 . The method of  claim 1  wherein J is 
       
         
           
           
               
               
           
         
         wherein each R 10  is independently selected from the group consisting of F, Cl, —OH, methyl, methoxy, ethoxy, propoxy, isopropoxy, and —OCOCH 3 ; and k is 0, 1, 2, or 3. 
       
     
     
         5 . The method of  claim 4  wherein each of X 1 , X 2 , X 3 , and X 4  is CH. 
     
     
         6 . The method of  claim 4  wherein X 1  is N, and each of X 2 , X 3 , and X 4  is CH. 
     
     
         7 . The method of  claim 4  wherein X 2  is N, and each of X 1 , X 3 , and X 4  is CH. 
     
     
         8 . The method of  claim 4  wherein X 3  is N, and each of X 1 , X 2 , and X 4  is CH. 
     
     
         9 . The method of  claim 4  wherein X 4  is N, and each of X 1 , X 2 , and X 3  is CH. 
     
     
         10 . The method of  claim 1  wherein Y is —W—(CH 2 ) n —R 1 . 
     
     
         11 . The method of  claim 10  wherein R 1  is —NR 2 R 3 . 
     
     
         12 . The method of  claim 11  wherein R 3  is selected from the group consisting of C 2 -C 10  alkyl, aryl, and cycloalkyl. 
     
     
         13 . The method of  claim 12  wherein R 3  is phenyl optionally substituted with one or more R 7 , wherein each R 7  is independently selected from the group consisting of hydroxyl, methoxy, —COOH, —O—(CH 2 ) m —OH, cyclopropylmethoxy, cyclopentylmethoxy, and isopropyl. 
     
     
         14 . The method of  claim 13  wherein each R 7  is independently selected from the group consisting of methoxy, —COOH, and —O—(CH 2 ) 3 —OH. 
     
     
         15 . The method of  claim 11  wherein R 3  is C 2 -C 10  alkyl, aryl, and cycloalkyl, where any atom of R 3  is optionally substituted with one or more R 7 . 
     
     
         16 . The method of  claim 1  wherein the compound is selected from any of: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
       or any pharmaceutically acceptable salt, tautomer, stereoisomer, or isotope thereof. 
     
     
         17 . The method of  claim 16  wherein the compound is selected from any of 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
       or any pharmaceutically acceptable salt, tautomer, stereoisomer, or isotope thereof. 
     
     
         18 . A method of  claim 1 , wherein the compound is selected from any of compounds shown below: 
       
         
           
                 
                 
               
                     
                 
                   Compound # 
                   Structure 
                 
                     
                 
                     
                 
                 
                 
               
                   3 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   4 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   5 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   66 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   67 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   68 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   69 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   70 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   71 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   72 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   73 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   74 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   75 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   76 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   77 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   78 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   79 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   80 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   81 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   83 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   85 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   86 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   87 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   92 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   93 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   118 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   166 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   167 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   168 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   169 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   170 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   171 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   172 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   173 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   174 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   175 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   176 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   177 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   178 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   179 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   181 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   182 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   184 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   185 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   186 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   187 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   188 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   189 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   190 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   191 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   192 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   193 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   194 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   195 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   196 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   197 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   198 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   199 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   200 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   201 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   202 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   203 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   204 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   205 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   206 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   207 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   208 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   209 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   210 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   211 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   212 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   213 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   214 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   215 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   216 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   218 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   219 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   220 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   221 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
             
                
                
                
               
               
                
               
            
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         19 . The method of  claim 1 , wherein the subject is a human being. 
     
     
         20 . The method of  claim 1 , wherein the MLK-mediated disease is selected from the group consisting of stroke, diabetes mellitus, hyperglycemia, retinopathy, nephropathy, neuropathy, ulcers, microangiopathies, macroangiopathies, gout, diabetic foot disease, insulin resistance, metabolic syndrome, hyperinsulinemia, hypertension, hyperuricemia, obesity, edema, dyslipidemia, chronic heart failure, atherosclerosis, peripheral inflammation, cancer, hepatitis, steatohepatitis, HIV associated neurocognitive disorder, HIV associated neuropathy, Alzheimer's disease, Parkinson's disease, multiple sclerosis, neurodegenerative disease, cirrhotic liver disease, and cirrhotic pancreatic disease. 
     
     
         21 . A method for treating a MLK-mediated disease comprising administering to a subject a therapeutically effective amount of a pharmaceutical composition comprising a compound having the structure of Formula I 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable isomer, isotope, enantiomer, or salt thereof, wherein J has a structure of 
       
       
         
           
           
               
               
           
         
       
       substituted with up to four R 10 ,
 or J has a structure of 
 
       
         
           
           
               
               
           
         
       
       optionally substituted with up to four R 10 ,
 each R 10  is independently selected from the group consisting of halo, alkyl, haloalkyl, alkoxy, haloalkoxy, —OH, and —OCOR 6 ; 
 X is NR 12  or S; 
 one of X 1 , X 2 , X 3 , and X 4  is N and the other three of X 1 , X 2 , X 3 , and X 4  are CH; 
 Y is —W—(CH 2 ) n —R 1 , 
 
       
         
           
           
               
               
           
         
         W is null, phenylene, or —NR 6 -phenylene, where the NR 6  is attached to the imidazopyridazine core structure of Formula I; 
         R 1  is —NR 2 R 3 , or piperazinyl, where the nitrogen atom of the piperazinyl is optionally substituted with alkyl or alkoxy; 
         R 2  is H or alkyl; 
         R 3  is selected from the group consisting of C 2 -C 10  alkyl, aryl, heteroaryl, cycloalkyl, and heterocycloalkyl, where any atom of R 3  is optionally substituted with one or more R 7 ; or R 2  and R 3  taken together with the N atom to which they are attached form a 3- to 7-membered heterocyclic ring optionally substituted with R 8 ; 
         R 4  is H or alkyl; 
         R 5  is H, alkyl, or NHR 9 ; 
         R 6  is H or alkyl; 
         each R 7  is independently alkyl, cycloalkyl, alkoxy, cycloalkoxy, cycloalkylalkyoxy, perhaloalkoxy, halo, oxo, —OH, hydroxyalkyl, —COOR 11 , or —O—(CH 2 ) m —OH; 
         R 8  is alkoxy, hydroxyalkyl, or COOR 11 ; 
         R 9  is H, alkyl, or cycloalkyl; 
         R 11  is H, or alkyl; 
         R 12  is H, or alkyl; 
         n is 0 or 1; 
         m is 1, 2, or 3; and 
         p is 1, 2, or 3; 
         with the proviso that if J is an unsubstituted benzothiophene, then R 3  is either aryl or heteroaryl, where any atom of R 3  is optionally substituted with one or more R 7 .

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