Methods and devices for obtaining and providing combination cell therapy

Abstract

The invention is directed to a therapeutic composition that includes a first cell preparation that includes isolated stromal cells of a first type and a second cell preparation that includes isolated stromal cells of a second type. The first and second cell preparations may include cultured cells. The stromal cells may include adipose stromal cells and bone marrow derived stromal cells. The therapeutic composition may include a blood component that includes one or more of platelet-poor plasma, concentrated platelet-poor plasma, platelet-rich plasma, and whole blood.

What is claimed is: 
     
         1 . A therapeutic composition comprising:
 a first cell preparation comprising isolated stromal cells of a first type;   a second cell preparation comprising isolated stromal cells of a second type; and   a blood component.   
     
     
         2 . The therapeutic composition according to  claim 1 , wherein the blood component is selected from the group consisting of platelet-poor plasma, concentrated platelet-poor plasma, platelet-rich plasma, whole blood, and combinations thereof. 
     
     
         3 . The therapeutic composition according to  claim 1 , wherein the isolated stromal cells of the first type and the second type are cultured cells. 
     
     
         4 . The therapeutic composition according to  claim 2 , wherein the isolated stromal cells of the first type, the stromal cells of the second type, and the blood component are derived from the same subject. 
     
     
         5 . The therapeutic composition according to  claim 1 , wherein the isolated stromal cells of the first type are adipose stromal cells and the isolated stromal cells of the second type are bone marrow derived stromal cells. 
     
     
         6 . The therapeutic composition according to  claim 1 , wherein the isolated stromal cells of the first type and the second type are able to differentiate into cells selected from the group consisting of: chondrocytes, endothelial cells, osteoblasts, myocytes, neural cells, glial cells, adipocytes, pericytes, cardiomyocytes, epithelial cells, fibroblasts, and combinations thereof. 
     
     
         7 . The therapeutic composition according to  claim 1  further comprising an additive selected from the group consisting of: a cytokine, bioactive material, scaffold, buffer, or combinations thereof.