Methods and materials for producing transgenic artiodactyls

Abstract

Swine animal models comprising a genomic disruption of an endogenous gene chosen from the group consisting of a Low-Density Lipoprotein Receptor gene LDLR, Duchene's Muscular Dystrophy (DMD) gene, and hairless gene (HR). Methods of preparing transfected cells useful for making a transgenic animal comprising exposing a first group of cells to a transfection agent and reseeding the group with additional cells that have not been exposed to the agent. The transgenic animals are useful for medical and scientific animal models of human diseases and conditions, as well as sources for cells, tissues, and biomaterials.

1 . A transgenic livestock animal or cell comprising a genomic knockout of a gene in an artiodactyl that provides for maintenance and/or development of hair or hair growth. 
     
     
         2 . The livestock animal or cell of  claim 1  being sheep, goat, or cattle. 
     
     
         3 . The livestock animal or cell of  claim 1  being pig, miniature pig, or Ossabaw pig. 
     
     
         4 . The livestock animal or cell of  claim 1  wherein the gene is a Hairless gene (HR). 
     
     
         5 . The livestock animal or cell of  claim 4  wherein exon 2 of HR is targeted for the knockout of HR. 
     
     
         6 . The livestock animal or cell of  claim 1  wherein the gene provides for maintenance and/or development of hair growth. 
     
     
         7 . The livestock animal or cell of  claim 1  wherein the gene provides for maintenance and/or development of hair. 
     
     
         8 . The livestock animal of  claim 1  having skin denuded of hair. 
     
     
         9 . A method of making a transgenic livestock animal or cell knocking out a gene in an artiodactyl that provides for maintenance and/or development of hair or hair growth. 
     
     
         10 . The method of  claim 9  comprising using recombinant Adeno-associated virus (rAAV) homologous recombination to knock out the gene. 
     
     
         11 . The method of  claim 9  wherein the gene is a Hairless gene (HR). 
     
     
         12 . The method of  claim 11  wherein exon 2 of HR is targeted for the knockout of HR. 
     
     
         13 . The method of  claim 9  comprising performing nuclear transfer of the cell to make the animal. 
     
     
         14 . Tissue isolated from the transgenic livestock of  claim 1 .