Novel Method for the cheap, efficient, and effective production of pharmaceutical and therapeutic api's intermediates, and final products

Abstract

The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular/modified/synthetic gene(s) of select enzymes are processed and inserted into an expression system (vector, cosmid, BAC, YAC, phage, etc.) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.

1 ) Process of biosynthesis of compounds of interest (Table 1); using a cheap and readily available starting materials in any organism or cell-free expression system (Table 2). 
     
     
         2 ) Process of genetically engineering micro-organisms that produce compounds of interest in large/industrial-scale quantities. 
     
     
         3 ) Process of altering genes in organisms to allow better yields for compounds of interest. 
     
     
         4 ) Process of genetically engineering food plants that are optimized for production of compounds of interest. 
     
     
         5 ) Using  s. cerevisaie, e coli, p. pastorisis, n. crassa, s. pombe, r. palmatum, c. longa, o. sativa, a. arborescens, a. terrus, c. sativa, s. griseus, s. erythera, s. coelicolor, s. toxytruini, s. cellulosum, p. floresceus, a. orientalis, s. livedans, a. vinelandii, b. subtilis, m. tuberculosis, m. xanthus, a. oryzae, a. niger, r. palmatum, h. serrata, r. rubra, l. erythrhizon, l. acetobutylicum, c. utilis, a. niger, c. glutamicum, b . spp., and other large-scale biotechnology organisms to biosynthesize a total pathway using cheap and readily available starting materials to make our compounds of interest. 
     
     
         6 ) Process of  claims 1  to  5 , wherein the products are those listed in table 1. 
     
     
         7 ) Process of  claims 1  to  6 , wherein the starting materials are those listed in table 2. 
     
     
         8 ) Process of  claims 1  to  7 , wherein certain steps are performed outside or inside an organism or cell free expression system using various techniques, while other steps may use biosynthesis techniques. 
     
     
         9 ) Producing compositions (e.g. tinctures, balms, capsules, tablets, concentrates, edibles, topicals) that use  claims 1  to  8 , whether they are classified as pharmaceuticals or not. 
     
     
         10 ) Producing compositions that contain combinations of certain compounds for certain therapeutic and non-therapeutic applications, as listed in table 1. 
     
     
         11 ) To  claim 10 , compositions that have added cofactors for better efficacy, absorption, etc, for our compounds of interest. These include terpenes, sequiterpenes, vitamins, and other cofactors. 
     
     
         12 ) cDNA sequences of enzymes and organisms in the pathways and processes listed in  FIGS. 1-7  for biosynthesis of compounds of interest using cheap and readily available starting materials, including mRNA's, tRNA's, vectors, and other genetic coding molecules needed for process of  claims 1  to  8 . 
     
     
         13 ) A method for the efficient and cheap biosynthesis of CBGA, THCA, CBDA, CBCA, CBDVA, THCVA, CBGVA, and CBCVA utilizing manipulation of any ERG, IDI, gal80p, upc2-1, HMGR, tHMGR, ALD6, DPP1, and ADH2 genes that result in exogenous sterol mutations for the uptake of exogenous sterols, more permeable cell membrane for easy inward flow of exogenous materials while easy outward flow of our compounds of interest, and carbon flux manipulation and blocking of other pathways for many fold higher yields in  S. Cerevisiae  and  P. Pastoris.