Platelet-Derived Growth Factor Compositions and Methods of Use Thereof

Abstract

A method for promoting growth of bone, periodontium, ligament, or cartilage in a mammal by applying to the bone, periodontium, ligament, or cartilage a composition comprising platelet-derived growth factor at a concentration in the range of about 0.1 mg/mL to about 1.0 mg/mL in a pharmaceutically acceptable liquid carrier and a pharmaceutically-acceptable solid carrier.

What is claimed is: 
     
         1 - 78 . (canceled) 
     
     
         79 . An implant material comprising a porous calcium sulfate having incorporated therein a liquid comprising platelet-derived growth factor (PDGF) at a concentration in the range of about 0.1 mg/mL to about 1.0 mg/mL in a buffer, wherein the calcium sulfate has a porosity greater than 40% and comprises particles in a range of about 100 microns to about 2000 microns in size. 
     
     
         80 . The implant material of  claim 79 , wherein the concentration of PDGF is about 0.3 mg/mL. 
     
     
         82 . The implant material of  claim 79 , wherein the buffer is sodium acetate buffer. 
     
     
         83 . A method of preparing an implant material comprising saturating a calcium sulfate material in a sterile liquid comprising platelet-derived growth factor (PDGF) at a concentration in the range of about 0.1 mg/mL to about 1.0 mg/mL, and wherein the calcium sulfate comprises particles in a range of about 100 microns to about 2000 microns in size, wherein the implant material is resorbable such that at least 80% of the calcium sulfate is resorbed within one year of being implanted. 
     
     
         84 . The method of  claim 79 , wherein the concentration of PDGF is about 0.3 mg/mL. 
     
     
         85 . The implant material of  claim 79 , wherein the PDGF is recombinant PDGF. 
     
     
         86 . The implant material of claim, wherein the recombinant PDGF is recombinant PDGF-BB. 
     
     
         81 . The implant material of  claim 79 , wherein the liquid comprises PDGF at a concentration in a range of about 0.25 mg/mL to about 0.75 mg/mL in a buffer. 
     
     
         82 . The implant material of  claim 79 , wherein the calcium sulfate comprises particles in a range of about 250 microns to about 1000 microns in size. 
     
     
         83 . The implant material of  claim 79 , wherein the implant material is resorbable such that at least 80% of the calcium sulfate is resorbed within one year of being implanted. 
     
     
         84 . The implant material of  claim 79 , wherein the incorporated liquid is adsorbed or absorbed to the calcium sulfate. 
     
     
         86 . The implant material of  claim 79  further comprising collagen. 
     
     
         87 . An implant material comprising a calcium sulfate having incorporated therein a liquid comprising platelet derived growth factor (PDGF) at a concentration in a range of about 0.1 mg/mL to about 1.0 mg/mL in a buffer, wherein the implant material is a composition having a porosity that facilitates cell migration into the composition and the calcium sulfate comprises particles in a range of about 100 microns to about 2000 microns in size, wherein the implant material is resorbable such that at least 80% of the calcium sulfate is resorbed within one year of being implanted, and wherein the implant material is flowable. 
     
     
         88 . The implant material of  claim 87 , wherein said porosity comprises macroporosity. 
     
     
         89 . The implant material of  claim 87 , wherein said calcium sulfate particles have a porosity greater than 40%. 
     
     
         90 . The implant material of  claim 87 , wherein the PDGF is recombinant PDGF. 
     
     
         91 . The implant material of  claim 90 , wherein the recombinant PDGF is recombinant PDGF-BB. 
     
     
         92 . The implant material of  claim 87 , wherein the liquid comprises PDGF at a concentration of about 0.3 mg/mL in a buffer. 
     
     
         93 . The implant material of  claim 87 , wherein the liquid comprises PDGF at a concentration in a range of about 0.25 mg/mL to about 0.75 mg/mL in a buffer. 
     
     
         94 . The implant material of  claim 87 , wherein the calcium sulfate comprises particles in a range of about 250 microns to about 1000 microns in size. 
     
     
         96 . The implant material of  claim 87 , wherein the incorporated liquid is adsorbed or absorbed to the calcium sulfate. 
     
     
         97 . The implant material of  claim 87  further comprising collagen. 
     
     
         98 - 108 . (canceled)