Antibody capable of binding to specific region of periostin, and method for measuring periostin using same

Abstract

The present invention provides a method and a reagent for measuring periostin contained in a sample with improved accuracy, a method for improving accuracy in measurement of periostin, and a method of testing for pulmonary fibrosis or interstitial pneumonia with improved accuracy. The antibody of the present invention binds to at least one region selected from the group consisting of an EMI region, an R1 region, an R2 region, and an R3 region of periostin or a cleavage product thereof. The method and the reagent for measuring periostin and the method for improving accuracy in periostin measurement of the present invention is characterized by detecting at least one region selected from the group consisting of an EMI region, an R1 region, an R2 region, and an R3 region of periostin. The method of testing for pulmonary fibrosis or interstitial pneumonia of the present invention includes the steps of a) measuring the amount or concentration of periostin in a sample derived from a subject, which measuring includes detecting at least one region selected from the group consisting of an EMI region, an R1 region, an R2 region, and an R3 region of periostin and b) comparing the amount or concentration of the periostin in the sample derived from the subject with an amount or concentration of periostin in a sample derived from a living body not suffering from pulmonary fibrosis and interstitial pneumonia.

1 . A reagent for measuring periostin or a cleavage product thereof contained in a sample, the reagent comprising an antibody that binds to at least one region of periostin or a cleavage product thereof,
 wherein the region is selected from the group consisting of an EMI region having an amino acid sequence consisting of SEQ ID NO: 4, an R1 region having an amino acid sequence consisting of SEQ ID NO: 6, an R2 region having an amino acid sequence consisting of SEQ ID NO: 8, and an R3 region having an amino acid sequence consisting of SEQ ID NO: 10, and wherein the antibody does not bind to periostin multimers.   
     
     
         2 . The reagent according to  claim 1 , wherein the antibody is a monoclonal antibody. 
     
     
         3 . The reagent according to  claim 2 , wherein an amino acid sequence of a heavy chain variable region of the antibody comprises the amino acid sequence set forth in SEQ ID NO: 16 and an amino acid sequence of a light chain variable region of the antibody comprises the amino acid sequence set forth in SEQ ID NO: 18. 
     
     
         4 . The reagent according to  claim 1 , wherein the antibody is a monoclonal antibody produced by a hybridoma selected from the group consisting a hybridoma cell line SS19D having Accession Number NITE BP-1068, a hybridoma cell line SS25A having Accession Number NITE BP-1285, and a hybridoma cell line SS27A having Accession Number NITE BP-1286. 
     
     
         5 . The reagent according to  claim 1 , further comprising a monoclonal antibody produced by a hybridoma selected from the group consisting of a hybridoma cell line SS16A having Accession Number NITE BP-1281, a hybridoma cell line SS18A having Accession Number NITE BP-1282, a hybridoma cell line SS19C having Accession Number NITE BP-1283 and a hybridoma cell line SS20A having Accession Number NITE BP-1284