Asarum total polysaccharides extract having antitussive activity and extraction method and use thereof

Abstract

The present invention discloses an Asarum total polysaccharide extract with antitussive activity, an extraction method thereof and an application in the preparation of a medicine for preventing and treating coughs. The present invention proves that the Asarum total polysaccharide extract has a significant effect of suppressing cough for the first time through a large number of pharmacodynamic tests, has the therapeutic effect approximate to codeine, has the effect of prolonging the cough latent period better than codeine, and also finds that the total Asarum polysaccharide extract has the effects of reducing the cough sensitivity and suppressing the airway inflammation for the first time, has excellent preventive and therapeutic effects on various types of coughs, and can be used for preparing the medicines for preventing and treating cough-related diseases.

1 . An application comprising  Asarum  total polysaccharide extract in the preparation of a medicine for preventing and treating coughs. 
     
     
         2 . The application according to  claim 1 , wherein the cough is chronic cough. 
     
     
         3 . The application according to  claim 1 , wherein the percentage content of polysaccharide in the  Asarum  total polysaccharide extract is 60 wt % to 80 wt %. 
     
     
         4 . A pharmaceutical composition for preventing and treating coughs, wherein the active ingredients comprise  Asarum  total polysaccharide extract. 
     
     
         5 . The pharmaceutical composition for preventing and treating coughs according to  claim 4 , wherein the formulations of the pharmaceutical composition are granules, tablets, capsules, pills, dripping pills, effervescent tablets, ointments, syrups, injections, oral liquid, mixtures, tinctures, sustained-release preparations, controlled-release preparations or targeting preparations. 
     
     
         6 . The pharmaceutical composition for preventing and treating coughs according to  claim 4 , wherein the percentage content of polysaccharide in the  Asarum  total polysaccharide extract is 60 wt % to 80 wt %. 
     
     
         7 . A method for extracting the  Asarum  total polysaccharide extract, comprising the following steps: using a polar solvent for the heat reflux extraction of roots and/or rhizomes of  Asarum;  decompressing, concentrating and drying the obtained material to obtain an  Asarum  total extract; dissolving the  Asarum  total extract in water to obtain an  Asarum  total extract solution;
 adding ethanol, wherein the volume percentage of the ethanol in the  Asarum  total extract solution is 60%-99%;   decompressing and filtering the solution;   using an ethanol solution for washing; and decompressing and drying to obtain the  Asarum  total polysaccharide extract.   
     
     
         8 . The method according to  claim 7 , wherein the polar solvent is water. 
     
     
         9 . The method according to  claim 7 , wherein the weight of the polar solvent is 5-20 times as much as that of the roots and/or rhizomes of  Asarum.    
     
     
         10 . The method according to  claim 7 , wherein the volume percentage of the ethanol solution for washing is 60%-100%. 
     
     
         11 . The method according to any of  claim 7 , wherein the  Asarum  is  Asarum Heterotropoides  Fr. Schmidt var.  Mandshuricum  (Maxim.) Kitag.,  Asarum sieboldii  Miq. var.  seoulense  Nakai or  Asarum sieboldii  Miq. 
     
     
         12 . The method according to  claim 11 , wherein the  Asarum  is  Asarum Heterotropoides  Fr. Schmidt var.  Mandshuricum  (Maxim.) Kitag. 
     
     
         13 . The  Asarum  total polysaccharide extract with antitussive activity obtained by the method according to  claim 7 .