Compositions for isolating a target analyte from a heterogeneous sample

Abstract

The invention generally relates to compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen.

1 - 20 . (canceled) 
     
     
         21 . A composition for isolating pathogen from a heterogeneous sample, the composition comprising:
 a plurality of sets of magnetic particles, members of different sets being conjugated to different antibodies that are specific for a pathogen; and   a buffer that reduces particle aggregation.   
     
     
         22 . The composition of  claim 21 , wherein each set is present at a concentration designed for detection of a specific pathogen in said sample. 
     
     
         23 . The composition of  claim 21 , wherein the particles are differently labeled. 
     
     
         24 . The composition of  claim 23 , wherein the label is an optical label. 
     
     
         25 . The composition of  claim 24 , wherein the optical label is a fluorescent label. 
     
     
         26 . The composition of  claim 21 , wherein the antibodies are monoclonal antibodies. 
     
     
         27 . The composition of  claim 21 , wherein the antibodies are polyclonal antibodies. 
     
     
         28 . The composition of  claim 21 , wherein the pathogen are gram positive bacteria. 
     
     
         29 . The composition of  claim 21 , wherein the pathogen are gram negative bacteria. 
     
     
         30 . The composition of  claim 21 , wherein the pathogen are gram positive and gram negative bacteria. 
     
     
         31 . The composition of  claim 21 , wherein the composition comprises a second antibody to which is attached. 
     
     
         32 . A plurality of magnetic particles within a buffer that reduces particle aggregation, wherein the plurality is differently functionalized so that the plurality of particles bind to a plurality of different pathogens in a heterogeneous sample. 
     
     
         33 . The particles of  claim 32 , wherein the particles are differently labeled. 
     
     
         34 . The particles of  claim 33 , wherein the label is an optical label. 
     
     
         35 . The particles of  claim 34 , wherein the optical label is a fluorescent label. 
     
     
         36 . The particles of  claim 32 , wherein the antibodies are monoclonal antibodies. 
     
     
         37 . The particles of  claim 32 , wherein the antibodies are polyclonal antibodies. 
     
     
         38 . The particles of  claim 32 , wherein the pathogen are gram positive bacteria. 
     
     
         39 . The particles of  claim 32 , wherein the pathogen are gram negative bacteria. 
     
     
         40 . The particles of  claim 32 , wherein the pathogen are gram positive and gram negative bacteria.