Preparation of Stabilized Catalase Enzymes Using Polyvinyl Alcohol

Abstract

There is provided a method of producing a stabilized catalase enzyme. In the method, a substrate is thoroughly mixed with phosphate borate and catalase, rinsed with water and the solids dried. The dried solid may be mixed with polyvinyl alcohol and dried for further stabilization. The stabilized powder may be mixed with various skin solutions (lotions, ointments and the like). The catalase enzyme can catalyze the reaction of peroxide to oxygen.

1 - 5 . (canceled) 
     
     
         6 . A microcrystalline cellulose adsorbed with a catalase enzyme and further comprising polyvinyl alcohol, wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol exhibits a decreased loss in catalase activity compared to a catalase enzyme not adsorbed on the substrate with the polyvinyl alcohol, wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol has an activity at 25° C. between 500 IU/g, and 1,000,000 IU/g, and wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol is stable for up to 16 weeks at 35° C. after thermal cycling at 40° C. for 72 hours and 55° C. for 6 hours. 
     
     
         7 . A skin hydrator lotion comprising lotion and the microcrystalline cellulose of  claim 6 . 
     
     
         8 . (canceled) 
     
     
         9 . The microcrystalline cellulose of  claim 6 , wherein the ratio of the microcrystalline cellulose to the catalase enzyme adsorbed on the microcrystalline cellulose is between 1 and 10. 
     
     
         10 . The microcrystalline cellulose of  claim 6 , wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol includes manganese atoms. 
     
     
         11 . The microcrystalline cellulose of  claim 6 , wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol has a molecular weight of less than 500,000 Daltons. 
     
     
         12 . The microcrystalline cellulose of  claim 6 , wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol is derived from fungus. 
     
     
         13 . The microcrystalline cellulose of  claim 12 , wherein the fungus is  Aspergillus niger.    
     
     
         14 . The microcrystalline cellulose of  claim 6 , wherein the polyvinyl alcohol has a molecular weight between 85,000 and 124,000 Daltons. 
     
     
         15 . The microcrystalline cellulose of  claim 6 , wherein the catalase enzyme adsorbed on the microcrystalline cellulose with the polyvinyl alcohol can decompose from above 60% to 91% hydrogen peroxide after thermal cycling when the ratio of the catalase enzyme to hydrogen peroxide is 1:1. 
     
     
         16 . The microcrystalline cellulose of  claim 6 , wherein the microcrystalline cellulose further comprises phosphate and borate.