Isolation and detection of cancer cells

Abstract

Provided are methods of using polycarbonate filters to isolate and detect cancer cells in a biological fluid, particularly biological fluids, such as urine, that contain very low concentrations of cancer cells. The characterization of the isolated cells for the presence or absence of cancer specific proteins is useful for cancer diagnosis and prognosis

1 . A method of detecting a cancer cell in a biological fluid suspected of containing the cancer cell, the method comprising:
 incubating the biological fluid with a reagent for fixing cells;   passing the biological fluid through a filter, such that if the biological fluid contains the cancer cell, the cancer cell is retained on the filter, wherein the filter comprises a polycarbonate substrate and a plurality of pores;   washing the filter;   incubating the filter with at least one detectable reagent that binds to the cancer cell to form a detectable complex;   washing the filter; and   detecting the detectable complex directly on the filter, wherein detecting the detectable complex indicates the presence of the cancer cell in the biological fluid.   
     
     
         2 . The method of  claim 2 , wherein the biological fluid is urine. 
     
     
         3 . The method of  claim 2 , wherein the cancer cell is a prostate cancer cell, a bladder cancer cell, a kidney cancer cell, a ureter cancer cell, or a urethra cancer cell. 
     
     
         4 . The method of  claim 1 , wherein the reagent for fixing cells comprises one or more alcohols. 
     
     
         5 . The method of  claim 4 , wherein the one or more alcohols comprise one or more of methyl alcohol, ethyl alcohol, and isopropyl alcohol. 
     
     
         6 . The method of  claim 1 , wherein each pore in the plurality of pores has a diameter of about 2 μm to 8 μm 
     
     
         7 . The method of  claim 1 , wherein the filter comprises a single layer of polycarbonate substrate and the pores pass straight through the filter. 
     
     
         8 . The method of  claim 1 , wherein the at least one detectable reagent is at least one antibody or at least one nucleic acid probe. 
     
     
         9 . The method of  claim 1 , wherein the at least one antibody binds to one of the following human proteins: an Ets Related Gene (ERG) protein, an alpha-methylacyl-CoA racemase (AMACR) protein, a prostate specific antigen (PSA) protein, a nucleolin protein, a phosphatase and tensin homolog (PTEN) protein, a phosphorylated Akt protein, a c-Myc protein, a RAF protein, a p63 protein, a prostate specific membrane antigen (PSMA) protein, a NKX3.1 protein, a high molecular weight cytokeratin, a SPARC protein, a SPINK1 protein, a SPOP protein, a BRAF protein, a p53 protein, a NCOA2 protein, a MAOA protein, a CAMK2N1 protein, COL3A1, CLDN8, HOXC6, TMEFF2, NPY, HPGD, BICD1, OR51E1 (PSGR), OR51E2 (PSGR2), NEFH, MSMB, CACNA1D, PLA2G7, MYO6, CRISP3, TWIST1, JAG1, PCGEM1, PMEPA1, LTF, and ETV1 or ETV4. 
     
     
         10 . The method of  claim 9 , wherein the at least one antibody binds to an ERG epitope formed by amino acids 42-66 of SEQ ID NO: 1. 
     
     
         11 . The method of  claim 9 , wherein the at least one antibody comprises an antibody that binds to a human ERG3 protein, an antibody that binds to a human AMACR protein, and an antibody that binds to a human PSA protein or a human NKX3.1 protein. 
     
     
         12 . The method of  claim 1 , wherein the at least one nucleic acid probe detects expression of one of the following human genes: the Ets Related Gene (ERG), alpha-methylacyl-CoA racemase (AMACR), prostate specific antigen (PSA), nucleolin gene, phosphatase and tensin homolog (PTEN), Akt, c-Myc, RAF, p63, prostate specific membrane antigen (PSMA), NKX3.1, a human gene encoding a high molecular weight cytokeratin, SPARC, SPINK1, SPOP, BRAF, p53, NCOA2, MAOA, CAMK2N1, COL3A1, CLDN8, HOXC6, TMEFF2, NPY, HPGD, BICD1, OR51E1 (PSGR), OR51E2 (PSGR2), NEFH, MSMB, CACNA1D, PLA2G7, MYO6, CRISP3, TWIST1, JAG1, PCGEM1, PMEPA1, LTF, and ETV1 or ETV4. 
     
     
         13 . The method of  claim 1 , wherein as few as 10 cancer cells can be detected in the biological fluid. 
     
     
         14 . The method of  claim 1 , wherein the method is capable of detecting at least about 70% of cancer cells in a sample containing 100 cancer cells. 
     
     
         15 . The method of  claim 1 , wherein the method does not include a centrifugation step. 
     
     
         16 - 23 . (canceled)