Lactobacillus-based ecoli inhibition

Abstract

A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal by providing to the animal a composition having 1×10 13 cfu of Lactobacillus probiotic microorganisms per kilogram of feed. When 1×10 4 viable cells of the Lactobacillus probiotic are added to 1×10 4 viable cells of Escherichia coli in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of Escherichia coli is reduced compared to a preparation of 1×10 4 viable cells of Escherichia coli mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of Lactobacillus probiotic.

1 .- 20 . (canceled) 
     
     
         21 . A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal comprising providing to an animal a composition comprising 1×10 13  cfu of  Lactobacillus animalis  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus animalis  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 41.24% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus animalis  probiotic. 
     
     
         22 . The method of  claim 21 , wherein when 1×10 4  viable cells of the  Lactobacillus animalis  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 42.17% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus animalis  probiotic. 
     
     
         23 . The method of  claim 21 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus agilis  probiotic microorganisms microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus agilis  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 72.88% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus agilis  probiotic. 
     
     
         24 . The method of  claim 23 , wherein when 1×10 4  viable cells of the  Lactobacillus agilis  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 67.61% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus agilis  probiotic. 
     
     
         25 . The method of  claim 21 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus reuteri  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 60.05% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         26 . The method of  claim 25 , wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 42.17% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         27 . The method of  claim 21 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus murinus  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus murinus  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 64.95% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus murinus  probiotic. 
     
     
         28 . The method of  claim 27 , wherein when 1×10 4  viable cells of the  Lactobacillus murinus  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 56.60% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus murinus  probiotic. 
     
     
         29 . The method of  claim 21 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus salivarius  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus salivarius  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 62.71% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus salivarius  probiotic. 
     
     
         30 . The method of  claim 29 , wherein when 1×10 4  viable cells of the  Lactobacillus salivarius  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 42.17% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus salivarius  probiotic. 
     
     
         31 . The method of  claim 29 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus reuteri  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 60.05% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         32 . The method of  claim 29 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus murinus  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus murinus  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 64.95% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus murinus  probiotic. 
     
     
         33 . The method of  claim 32 , wherein the composition further comprises 1×10 13  cfu of  Lactobacillus reuteri  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 60.05% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         34 . The method of  claim 21 , wherein the composition further comprises  Enterococcus faecium, Bacillus licheniformis, Lactococcus lactis, Bacillus subtilis, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium bifudum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermophilum, Lactobacillus acidophilus, Lactobacillus agilis, Lactobacillus alactosus, Lactobacillus alimentarius, Lactobacillus amylophilus, Lactobacillus amylovorans, Lactobacillus amylovorus, Lactobacillus animalis, Lactobacillus batatas, Lactobacillus bavaricus, Lactobacillus bifermentans, Lactobacillus bifidus, Lactobacillus brevis, Lactobacillus buchnerii, Lactobacillus bulgaricus, Lactobacillus catenaforme, Lactobacillus casei, Lactobacillus cellobiosus, Lactobacillus collinoides, Lactobacillus confusus, Lactobacillus coprophilus, Lactobacillus coryniformis, Lactobacillus corynoides, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus desidiosus, Lactobacillus divergens, Lactobacillus enterii, Lactobacillus farciminis, Lactobacillus fermentum, Lactobacillus frigidus, Lactobacillus fructivorans, Lactobacillus fructosus, Lactobacillus gasseri, Lactobacillus halotolerans, Lactobacillus helveticus, Lactobacillus heterohiochii, Lactobacillus hilgardii, Lactobacillus hordniae, Lactobacillus inulinus, Lactobacillus jensenii, Lactobacillus jugurti, Lactobacillus kandleri, Lactobacillus kefir, Lactobacillus lactis, Lactobacillus leichmannii, Lactobacillus lindneri, Lactobacillus malefermentans, Lactobacillus mali, Lactobacillus maltaromicus, Lactobacillus minor, Lactobacillus minutus, Lactobacillus mobilis, Lactobacillus murinus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus pseudoplantarum, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus rogosae, Lactobacillus tolerans, Lactobacillus torquens, Lactobacillus ruminis, Lactobacillus sake, Lactobacillus salivarius, Lactobacillus sanfrancisco, Lactobacillus sharpeae, Lactobacillus sobrius, Lactobacillus trichodes, Lactobacillus vaccinostercus, Lactobacillus viridescens, Lactobacillus vitulinus, Lactobacillus xylosus, Lactobacillus yamanashiensis, Lactobacillus zeae, Pediococcus acidlactici, Pediococcus pentosaceus, Streptococcus cremoris, Streptococcus diacetylactis, Streptococcus faecium, Streptococcus intermedius, Streptococcus lactis, Streptococcus thermophilus, Propionibacterium freudenreichii, Propionibacterium acidipropionici, Propionibacterium jensenii, Propionibacterium thoenii, Propionibacterium cyclohexanicum, Propionibacterium granulosum, Propionibacterium microaerophilum, Propionibacterium propionicum, Propionibacterium acnes, Propionibacterium australiense, Propionibacterium avidum  or a combination thereof. 
     
     
         35 . A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal comprising providing to an animal a composition comprising 1×10 13  cfu of  Lactobacillus reuteri  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 60.05% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         36 . The method of  claim 35 , wherein when 1×10 4  viable cells of the  Lactobacillus reuteri  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 42.17% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus reuteri  probiotic. 
     
     
         37 . A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal comprising providing to an animal a composition comprising 1×10 13  cfu of  Lactobacillus murinus  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus murinus  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 64.95% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus murinus  probiotic. 
     
     
         38 . The method of  claim 37 , wherein when 1×10 4  viable cells of the  Lactobacillus murinus  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 56.60% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus murinus  probiotic. 
     
     
         39 . A method of reducing growth of pathogenic bacteria in a gastrointestinal tract of an animal comprising providing to an animal a composition comprising 1×10 13  cfu of  Lactobacillus salivarius  probiotic microorganisms per kilogram of feed, wherein when 1×10 4  viable cells of the  Lactobacillus salivarius  probiotic are added to 1×10 4  viable cells of  Escherichia coli  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Escherichia coli  is reduced by at least 62.71% compared to a preparation of 1×10 4  viable cells of  Escherichia coli  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus salivarius  probiotic. 
     
     
         40 . The method of  claim 39 , wherein when 1×10 4  viable cells of the  Lactobacillus salivarius  probiotic are added to 1×10 4  viable cells of  Salmonella  in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours, the growth of  Salmonella  is reduced by at least 42.17% compared to a preparation of 1×10 4  viable cells of  Salmonella  mixed in a solution of MRS broth in a test tube and incubated in a water bath at 37° C. for six hours without the addition of  Lactobacillus salivarius  probiotic.