Multilayered cell culture apparatus

Abstract

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

In the claims: 
     
         1 . A cell growth apparatus comprising:
 a plurality of cell growth chambers, each having a gas permeable, liquid impermeable surface and an opposing surface; and   at least one tracheal chamber in communication with at least one gas permeable, liquid impermeable surface of at least one cell growth chamber.