US2017095594A1PendingUtilityA1

Phospholipid Reduction in Biological Tissue

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Assignee: MEDTRONIC INCPriority: Feb 24, 2014Filed: Dec 21, 2016Published: Apr 6, 2017
Est. expiryFeb 24, 2034(~7.6 yrs left)· nominal 20-yr term from priority
Inventors:Laura MckinleyMonique PoonWill Wei WangBenjamin Wong
A61L 2430/40A61L 27/3625A61L 2400/02A61L 27/3604A61L 27/3687A61L 2430/20
45
PatentIndex Score
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Claims

Abstract

A method of reducing phospholipid concentration in biological tissue. The biological tissue is immersed in an isopropyl alcohol solution for an effective period of time. After the effective period of time, the biological tissue has a phospholipid concentration that is at least 10 percent by weight less than an initial phospholipid concentration of the biological tissue.

Claims

exact text as granted — not AI-modified
1 - 21 . (canceled) 
     
     
         22 . A method of reducing phospholipid concentration in biological tissue comprising:
 preparing an organic solvent solution by mixing about 5 to about 50 percent by weight of isopropyl alcohol with water; and   prior to crosslinking of the biological tissue, immersing the biological tissue in the organic solvent solution for an effective period of time, wherein after the effective period of time, the biological tissue has a phospholipid that is at least 10 percent less than an initial phospholipid concentration of the biological tissue.   
     
     
         23 . The method of  claim 22 , wherein the organic solvent solution further comprises a buffer. 
     
     
         24 . The method of  claim 22 , and further comprising maintaining the organic solvent solution at a temperature of between about 4° C. and 10° C. while the biological tissue is immersed in the organic solvent solution. 
     
     
         25 . The method of  claim 22 , wherein the effective period of time is between about 12 hours and about 72 hours. 
     
     
         26 . The method of  claim 22 , and further comprising replacing at least a portion of the organic solvent solution while the biological tissue is immersed in the organic solvent solution. 
     
     
         27 . The method of  claim 22 , and further comprising agitating at least one of the biological tissue and the organic solvent solution while the biological tissue is immersed in the organic solvent solution. 
     
     
         28 . A method of reducing phospholipid concentration in biological tissue comprising:
 preparing an organic solvent solution by mixing about 5 to about 50 percent by weight of acetone with water; and   prior to crosslinking of the biological tissue, immersing the biological tissue in the organic solvent solution for an effective period of time, wherein after the effective period of time, the biological tissue has a phospholipid that is at least 10 percent less than an initial phospholipid concentration of the biological tissue.   
     
     
         29 . The method of  claim 28 , wherein the organic solvent solution further comprises a buffer. 
     
     
         30 . The method of  claim 28 , and further comprising maintaining the organic solvent solution at a temperature of between about 4° C. and 10° C. while the biological tissue is immersed in the organic solvent solution. 
     
     
         31 . The method of  claim 28 , wherein the effective period of time is between about 12 hours and about 72 hours. 
     
     
         32 . The method of  claim 28 , and further comprising replacing at least a portion of the organic solvent solution while the biological tissue is immersed in the organic solvent solution. 
     
     
         33 . The method of  claim 28 , and further comprising agitating at least one of the biological tissue and the organic solvent solution while the biological tissue is immersed in the organic solvent solution. 
     
     
         34 . A method of reducing phospholipid concentration in biological tissue comprising:
 preparing an organic solvent solution by mixing about 5 to about 50 percent by weight of ethanol with water; and   prior to crosslinking of the biological tissue, immersing the biological tissue in the organic solvent solution for an effective period of time, wherein after the effective period of time, the biological tissue has a phospholipid that is at least 10 percent less than an initial phospholipid concentration of the biological tissue.   
     
     
         35 . The method of  claim 34 , wherein the organic solvent solution further comprises a buffer. 
     
     
         36  The method of  claim 34 , and further comprising maintaining the organic solvent solution at a temperature of between about 4° C. and 10° C. while the biological tissue is immersed in the organic solvent solution. 
     
     
         37 . The method of  claim 34 , wherein the effective period of time is between about 12 hours and about 72 hours. 
     
     
         38 . The method of  claim 34 , and further comprising replacing at least a portion of the organic solvent solution while the biological tissue is immersed in the organic solvent solution. 
     
     
         39 . The method of  claim 34 , and further comprising agitating at least one of the biological tissue and the organic solvent solution while the biological tissue is immersed in the organic solvent solution.

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