Dna sequencing by nanopore using modified nucleotides

Abstract

This invention provides a process for sequencing single-stranded DNA or RNA by employing a nanopore and modified nucleotides.

1 . A method for determining the nucleotide sequence of a single-stranded DNA comprising the steps of:
 (a) passing the single-stranded DNA through a pore of suitable diameter by applying an electric field to the DNA, wherein at least each A or each G residue and at least each C, each T or each U residue comprises a modifying group bound to its respective base so that each type of nucleotide in the DNA has an electronic signature which is distinguishable from the electronic signature of each other type of nucleotide in the DNA;   (b) for each nucleotide of the DNA which passes through the pore, determining an electronic signature for such nucleotide; and   (c) comparing each electronic signature determined in step (b) with electronic signatures corresponding to each of A, G, C and T modified as per the nucleotides in the single-stranded DNA, so as to determine the identity of each such nucleotide,   
       thereby determining the nucleotide sequence of the single-stranded DNA. 
     
     
         2 - 17 . (canceled)