Method for producing a recombinant peptide and resultant peptide

Abstract

The invention relates to peptides with the following general formula: A-Thr-Lys-Pro-B-C-D-X, where: A—0, Met, Met (O), Thr, Ala, His, Phe, Lys, Gly B—0, Gly, Asp, Trp, Gln, Asn, Tyr, Pro, Arg C—0, Arg, Phe, Tyr, Gly, His, Pro, Lys D—0, Val, Gly, Tyr, Trp, Phe, His X—OH, OCH 3 , NH 2 , where 0 is no amino acid residue, provided that if A≠0, then B and/or C and/or D≠0, if B≠0, then C and/or D≠0, excluding the peptides Phe-Thr-Lys-Pro-Gly (SEQ ID NO: 1), Thr-Lys-Pro-Pro-Arg (SEQ ID NO: 2), Thr-Lys-Pro-Arg-Gly (SEQ ID NO: 3).

1 - 2 . (canceled) 
     
     
         3 . A pharmaceutical composition comprising a peptide of formula:
   A-Thr-Lys-Pro-B-C-D-X,   
       wherein:
 A is absent, Met, Met(O), Thr, Ala, His, Phe, Lys; 
 B is absent, Gly, Asp, Trp, Gln, Asn, Tyr, Pro, Arg; 
 C is absent, Arg, Phe, Tyr, Gly, His, Pro; 
 D is absent, Val, Tyr, Trp, Phe, His; 
 X is a carboxy terminal OH, OCH 3 , or NH 2  group, 
 
       provided that (i) when A is present, at least one of B, C, or D is also present, or when B is present, then C and/or D is also present and (ii) the peptide is not a tetrapeptide and is not Thr-Lys-Pro-X (SEQ ID NO: 5), Phe-Thr-Lys-Pro-Gly-X (SEQ ID NO: 1), Thr-Lys-Pro-Pro-Arg-X (SEQ ID NO: 2), Thr-Thr-Lys-Pro-Arg-X (SEQ ID NO: 607), Lys-Thr-Lys-Pro-Arg-X (SEQ ID NO: 772), Thr-Lys-Pro-Gly-Pro-X (SEQ ID NO: 460), Thr-Lys-Pro-Gly-Arg-X (SEQ ID NO: 456), Thr-Lys-Pro-Arg-Tyr (SEQ ID NO: 494), or Thr-Lys-Pro-Arg-Gly-X (SEQ ID NO: 3), wherein said peptide is present in the composition in an amount effective for stimulating sexual or reproductive function. 
     
     
         4 . The pharmaceutical composition of  claim 3 , wherein said peptide is present in the composition in the form of a salt. 
     
     
         5 . The pharmaceutical composition of  claim 4 , wherein said peptide salt is produced by chemical synthesis. 
     
     
         6 . The pharmaceutical composition of  claim 5 , wherein said chemical synthesis includes a step of ion-exchange resin replacing a counter-ion. 
     
     
         7 . A method for stimulating sexual or reproductive function in a mammal in need thereof comprising administering to said mammal the pharmaceutical composition of  claim 3 . 
     
     
         8 . The method of  claim 7 , wherein the mammal has a reproductive or sexual dysfunction. 
     
     
         9 . A method for treating a reproductive or sexual dysfunction in a mammal in need of such treatment comprising administering to said mammal the pharmaceutical composition of  claim 3 . 
     
     
         10 . A method for stimulating sexual or reproductive function in a mammal in need thereof comprising administering to said mammal the pharmaceutical composition of  claim 4 . 
     
     
         11 . The method of  claim 10 , wherein the mammal has a reproductive or sexual dysfunction. 
     
     
         12 . A method for treating a reproductive or sexual dysfunction in a mammal in need of such treatment comprising administering to said mammal the pharmaceutical composition of  claim 4 . 
     
     
         13 . A method for stimulating sexual or reproductive function in a mammal in need thereof comprising administering to said mammal a pharmaceutical composition comprising a peptide of formula A-Thr-Lys-Pro-B-C-D-X, wherein:
 A is absent, Met, Met(O), Thr, Ala, His, Phe, Lys, Gly;   B is absent, Gly, Asp, Trp, Gln, Asn, Tyr, Pro, Arg;   C is absent, Arg, Phe, Tyr, Gly, His, Pro, Lys;   D is absent, Val, Gly, Tyr, Trp, Phe, His;   X is a carboxy terminal OH, OCH 3 , or NH 2  group,   
       provided that (i) when A is present, at least one of B, C, or D is also present, or when B is present, then C and/or D is also present and (ii) the peptide is not a tetrapeptide. 
     
     
         14 . The method of  claim 13 , wherein said peptide is present in the composition in the form of a salt. 
     
     
         15 . The method of  claim 13 , wherein the mammal has a reproductive or sexual dysfunction. 
     
     
         16 . A method for treating a reproductive or sexual dysfunction in a mammal in need of such treatment comprising administering to said mammal a pharmaceutical composition comprising a peptide of formula A-Thr-Lys-Pro-B-C-D-X, wherein:
 A is absent, Met, Met(O), Thr, Ala, His, Phe, Lys, Gly;   B is absent, Gly, Asp, Trp, Gln, Asn, Tyr, Pro, Arg;   C is absent, Arg, Phe, Tyr, Gly, His, Pro, Lys;   D is absent, Val, Gly, Tyr, Trp, Phe, His;   X is a carboxy terminal OH, OCH 3 , or NH 2  group,   
       provided that (i) when A is present, at least one of B, C, or D is also present, or when B is present, then C and/or D is also present and (ii) the peptide is not a tetrapeptide. 
     
     
         17 . The method of  claim 16 , wherein said peptide is present in the composition in the form of a salt.