Method of culturing antrodia cinnamomea with high triterpenoids

Abstract

A method of culturing Antrodia cinnamomea with high Triterpenoids includes mixing a malt extract, glucose, peptone, agar, and a mushroom extract with distilled water to obtain a culture medium; providing the culture medium to a high-pressure sterilization machine for sterilization to obtain a sterilized culture medium; providing the sterilized culture medium to an incubator in a disinfection environment to obtain a solidified culture medium after a predetermined time; transplanting Antrodia cinnamomeas strains to the solidified culture medium at separated positions; and exposing the solidified culture medium under a beam.

1 . A method of culturing  Antrodia cinnamomea,  comprising the steps of:
 mixing a malt extract, glucose, peptone, agar, and a mushroom's extract with distilled water to obtain a culture medium, the culture medium including 1%-2% w/v malt extract, 1%-2% w/v glucose, 0.1%-0.2% w/v peptone, 1%-2% w/v agar, and 0.1%-0.2% w/v mushroom extract, the mushroom extract being a concentrated solution of  Flammulina velutipes, Auricularia auricula - judae, Tremella fuciformis,  or  Lentinus edodes  extract;   placing the culture medium into a high-pressure sterilization machine at a pressure of 1 kg/cm 2 , and 120° C.-121° C. for 25-30 minutes to obtain a sterilized culture medium;   placing the sterilized culture medium into a container in an incubator in a disinfection environment to cool the sterilized culture medium and to obtain a solidified culture medium;   inoculating  Antrodia cinnamomea  onto the solidified culture medium at separated positions; and   exposing the solidified culture medium under a red light with a wavelength of 620-625 nm and an illuminance of 400-600 Lux for thirty days at 25° C.-30° C.,   thereby increasing the concentration of Triterpenoids in the fruiting bodies of  Antrodia cinnamomeas.      
     
     
         2 . The method of  claim 1 , wherein the culture medium is put in a glass container. 
     
     
         3 - 4 . (canceled) 
     
     
         5 . The method of  claim 1 , wherein the high-pressure sterilization machine is a high-pressure sterilization caldron. 
     
     
         6 . The method of  claim 1 , wherein the sterilized culture medium is put in an incubator with a diameter of 90 mm and a height of 10 mm, and the depth of the sterilized culture medium in the incubator is 1 mm. 
     
     
         7 . (canceled) 
     
     
         8 . The method of  claim 1 , wherein the  Antrodia cinnamomea  is inoculated at a top, a bottom, a right, a left, and a center of the solidified culture medium, and an interval between the neighboring strains is 20 mm. 
     
     
         9 - 10 . (canceled)