Use of a Polyanionic Composition

Abstract

Disclosed herein is a use of a composition, comprising a non-toxic polyanionic material or a salt thereof to dissociate a polymeric membrane. In addition, a method of dissociating a polymeric membrane is also presented, the method comprising the steps of providing a polymeric membrane; and dissociating the polymeric membrane by adding a composition comprising a non-toxic polyanionic material to the polymeric membrane.

1 . A composition comprising a non-toxic polyanionic material or a salt thereof and a polymeric membrane, wherein the non-toxic polyanionic material is a polysaccharide sulfate, a poly(sodium styrene sulfonate) or a polyacrylic acid or a salt thereof, provided that the polysaccharide sulfate is not a glycosoaminoglycan. 
     
     
         2 . The composition of  claim 1 , wherein the polymeric membrane is a capsule that encapsulates a substance or is a barrier that blocks free movement of a substance. 
     
     
         3 . The composition of  claim 1 , wherein the polymeric membrane comprises sodium cellulose sulfate and polydiallyldimethylammonium chloride (pDADMAC). 
     
     
         4 . The composition of  claim 2 , wherein the substance is selected from one or more of a member of the group consisting of cells, proteins, biocompatible particles, and chemical compounds. 
     
     
         5 . The composition of  claim 1 , wherein the polyanionic material or a salt thereof has a number average molecular weight of from 4,000 to 20,000 Daltons. 
     
     
         6 . The composition of  claim 1 , wherein the polyanionic material or salt thereof is a polysaccharide sulfate. 
     
     
         7 . The composition of  claim 6 , wherein the polysaccharide sulfate is one or more of a member of the group consisting of dextran sulfate and cellulose sulfate with a number average molecular weight of from 4,000 to 20,000 Daltons and salts thereof. 
     
     
         8 . The composition of  claim 1 , wherein the non-toxic polyanionic material or a salt thereof is an anti-clumping agent comprising dextran sulfate. 
     
     
         9 . The composition of  claim 1 , wherein the non-toxic polyanionic material or a salt thereof comprises polysaccharide sulfate in a concentration of from 0.1 mg/mL to 100 mg/mL. 
     
     
         10 . A method of dissociating a polymeric membrane comprising the steps of:
 (a) providing a polymeric membrane; and   (b) dissociating the polymeric membrane by adding a composition comprising a non-toxic polyanionic material to the polymeric membrane,   wherein the non-toxic polyanionic material is a polysaccharide sulfate, a poly(sodium styrene sulfonate) or a polyacrylic acid or a salt thereof, provided that the polysaccharide sulfate is not a glycosoaminoglycan.   
     
     
         12 . The method of  claim 10 , wherein the polymeric membrane is placed in a liquid medium in step (a), wherein the composition comprising the non-toxic polyanionic material is added to the liquid medium to provide a concentration of non-toxic polyanionic material of from 0.01 to 30 mg/mL. 
     
     
         13 . The method of  claim 10 , wherein the cells are kept in a cell releasing medium for from 1 minute to 16 hours. 
     
     
         14 . The method of  claim 10 , wherein the polyanionic material or a salt thereof has a number average molecular weight of from 4,000 to 20,000 Daltons. 
     
     
         15 . The method of  claim 10 , wherein the polyanionic material or salt thereof is a polysaccharide sulfate, wherein:
 (a) each saccharide unit of the polysaccharide sulfate contains from 1 to 3 sulfate groups; and/or   (b) the polysaccharide sulfate is cellulose sulfate or dextran sulfate with a number average molecular weight of from 4,000 to 20,000 Daltons and salts thereof.   
     
     
         16 . A composition comprising a non-toxic polyanionic material or a salt thereof and a dissociated polymeric membrane, wherein the non-toxic polyanionic material is a polysaccharide sulfate, a poly(sodium styrene sulfonate) or a polyacrylic acid or a salt thereof, provided that the polysaccharide sulfate is not a glycosoaminoglycan. 
     
     
         17 . The composition of  claim 16 , wherein the non-toxic polyanionic material or a salt thereof has a number average molecular weight of from 4,000 to 20,000 Daltons. 
     
     
         18 . The composition of  claim 16 , wherein the non-toxic polyanionic material or salt thereof is a polysaccharide sulfate. 
     
     
         19 . The composition of  claim 16 , wherein the non-toxic polyanionic material or salt thereof is a polysaccharide sulfate comprising dextran sulfate and cellulose sulfate with a number average molecular weight of from 4,000 to 20,000 Daltons and salts thereof. 
     
     
         20 . The composition of  claim 16 , wherein the non-toxic polyanionic material or a salt thereof comprises polysaccharide sulfate in a concentration of from 0.1 mg/mL to 100 mg/mL.