Promoters for Regulating Expression in Plants

Abstract

Isolated nucleic acid molecules capable of regulating expression in plants, as well as expression [cassettes, vectors and transgenic plants comprising the same are provided.

1 . An isolated nucleic acid molecule capable of regulating expression in plants selected from the list comprising
 i) a nucleic acid molecule comprising a nucleic acid sequence described by SEQ ID NOS: 5, 35, 36, 37, or 38,   ii) a fragment of at least 250 consecutive bases of a molecule comprising a nucleic acid sequence described by any of SEQ ID NOS: 5, 35, 36, 37, or 38,   iii) a nucleotide molecule with a sequence identity of at least 60% to a transcription regulating nucleotide molecule comprising a nucleic acid sequence described by any of SEQ ID NO NOS: 5, 35, 36, 37, or 38, and   iv) a nucleotide molecule of at least 250 consecutive bases with a sequence identity of at least 60% to a transcription regulating nucleotide molecule comprising a nucleic acid sequence described by any of SEQ ID NOS: 5, 35, 36, 37, or 38,   v) a nucleotide molecule of at least 250 bases capable of hybridizing under conditions equivalent to hybridization in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C. to a transcription regulating nucleotide molecule comprising a nucleic acid sequence described by any of SEQ ID NOS: 5, 35, 36, 37, or 38, or the complement thereof;   vi) a nucleotide molecule of at least 250 bases capable of hybridizing under conditions equivalent to hybridization in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C. to a nucleic acid comprising 250 or more consecutive nucleotides of a transcription regulating nucleotide molecule comprising a nucleic acid sequence described by any of SEQ ID NOS: 5, 35, 36, 37, or 38, or the complement thereof; and   vii) a nucleotide molecule which is the complement or reverse complement of any of the previously mentioned nucleotide molecules under i) to vi).   
     
     
         2 . (canceled) 
     
     
         3 . (canceled) 
     
     
         4 . The isolated nucleic acid molecule of  claim 1 , wherein the sequences specified under ii), iii), iv), v), vi) and vii) are capable of modifying transcription in a plant or part thereof. 
     
     
         5 . The isolated nucleic acid molecule of  claim 1 , wherein the sequences specified under ii), iii), iv), v), vi) and vii) have substantially the same transcription regulating activity as a transcription regulating nucleotide molecule comprising the nucleic acid sequence of SEQ ID NO: 5. 
     
     
         6 . An expression cassette for regulating expression in plants comprising
 a) at least one isolated nucleic acid molecule capable of regulating expression in plants as defined in  claim 1 ,   and functionally linked thereto   b) at least one nucleic acid molecule which is heterologous in relation to said isolated nucleic acid molecule capable of regulating expression in plants.   
     
     
         7 . A vector comprising the isolated nucleic acid molecule of  claim 1 . 
     
     
         8 . A transgenic host cell or non-human organism comprising the isolated nucleic acid sequence of  claim 1 . 
     
     
         9 . A transgenic plant or plant cell comprising the isolated nucleic acid sequence of  claim 1 . 
     
     
         10 . The transgenic plant or plant cell of  claim 9 , wherein said plant or plant cell is from a dicotyledonous plant. 
     
     
         11 . (canceled) 
     
     
         12 . A method for the production of an expression cassette comprising the steps of:
 a. providing an isolated nucleic acid molecule of  claim 1 ; and   b. functionally linking said isolated nucleic acid molecule to a nucleic acid molecule heterologous to said isolated nucleic acid molecule.   
     
     
         13 . A method for the production of a transgenic plant comprising the steps of:
 a. providing the expression cassette of  claim 6 ;   b. transforming said expression cassette into a plant part or plant cell; and   c. regenerating a plant from said transformed plant part or plant cell.   
     
     
         14 . A vector comprising the expression cassette of  claim 6 . 
     
     
         15 . A transgenic host cell or non-human organism comprising the expression cassette of  claim 6 . 
     
     
         16 . A transgenic plant or plant cell comprising the expression cassette of  claim 6 . 
     
     
         17 . A method for the production of a transgenic plant comprising the steps of:
 a. providing the vector of  claim 7 ;   b. transforming said vector into a plant part or plant cell; and   c. regenerating a plant from said transformed plant part of plant cell.