Oxazoline Polymer Compositions and Use Thereof

Abstract

Compositions comprising an oxazoline polymer and optional linkers to carry a variety of molecules.

1 . - 24 . (canceled) 
     
     
         25 . A functionalized polyoxazoline (POX) polymer comprising the formula, I n -P-L-M-B, wherein
 I is an initiator comprising a first functional group,   n≧1,   P is a linear POX polymer,   L is a first linker comprising at least two amine groups, at least two imino (—NH—) groups or at least an amino group and an imino group; wherein L is attached to P by one of said at least two amine groups, by one of said at least two imino (—NH—) groups or by one of said at least an amino group and an imino group,   M is a second linker attached to L, M comprising a carbodiimide (CDI), a CDI-functionalized molecule, glycidol, succinic anhydride, an amidoamine, a linear polyamidoamine or a heterofunctional molecule, wherein M comprises a second functional group, and   B is a bioactive material attached to said second functional group of M.   
     
     
         26 . The polymer of  claim 25 , wherein B comprises an antibody or antigen-binding portion thereof 
     
     
         27 . The polymer of  claim 25 , wherein said bioactive material comprises a member of a binding pair (BP), a biologically active molecule (BAM), a pharmaceutically active agent (PAA) or combination thereof. 
     
     
         28 . The polymer of  claim 27 , wherein said PAA comprises a small molecule drug, a large molecule drug or derivative thereof. 
     
     
         29 . The polymer of  claim 28 , wherein said large molecule drug comprises a polypeptide or a polynucleotide. 
     
     
         30 . The polymer of  claim 28 , wherein said small molecule drug comprises a paclitaxel, a gemcitabine, a camptothecin or a derivative thereof. 
     
     
         31 . The polymer of  claim 25 , wherein B comprises a targeting moiety. 
     
     
         32 . The polymer of  claim 31 , wherein said targeting moiety comprises an antibody or an antigen-binding portion thereof. 
     
     
         33 . The polymer of  claim 25 , wherein said POX polymer comprises a poly(unsubstituted oxazoline) or a poly(substituted oxazoline). 
     
     
         34 . The polymer of  claim 33 , wherein said poly(substituted oxazoline) comprises a poly(2-methyloxazoline), a poly(2-ethyloxazoline), a poly(2-propyloxazoline), a poly(2-butyloxazoline) or a combination thereof. 
     
     
         35 . The polymer of  claim 25 , wherein L comprises an entire or a portion of ammonia, ethylenediamine, piperazine, 1,4,7,10-tetraazacyclododecane, tris(2-aminoethyl)amine, 4-(aminomethyl)piperidine, 1,3-diaminopropane, 2,2′-(ethylenedioxy)bis(ethylamine), 1,11-diamino-3,6,9-trioxaundecane, diethylenetriamine, hexamethylenediamine, triethylenetetramine or tris(2-aminoethyl)amine or 1,8-diaminooctane. 
     
     
         36 . The polymer of  claim 25 , wherein said second functional group of M comprises —OH, —COOH, an ester, an amide or a maleimide. 
     
     
         37 . The polymer of  claim 25 , wherein I is bonded covalently to P; and L is bonded covalently to M. 
     
     
         38 . The polymer of  claim 25 , wherein B is linked to M through a hydrolytically stable or unstable linkage. 
     
     
         39 . The polymer of  claim 25 , wherein said initiator comprises an alkyl, an aryl, an ester or an amide. 
     
     
         40 . An assay polymer comprising the polymer of  claim 25  and a detectable reporter.