Compositions of and methods for cancellous bone matrix

Abstract

A bone repair composition including a cancellous bone matrix with cortical and/or cancellous bone particles loaded therein; and a kit comprising the cancellous bone matrix and the cortical and/or cancellous bone particles.

1 . A bone repair composition, comprising: a cancellous bone matrix; and demineralized bone particles. 
     
     
         2 . The bone repair composition of  claim 1 , further comprising an excipient. 
     
     
         3 . The bone repair composition of  claim 2 , wherein the excipient solution is selected from the group consisting of glycerol, ethanol, lecithin, sucrose, and combinations thereof. 
     
     
         4 . The bone repair composition of  claim 1 , further comprising an additive selected from the group consisting of bone marrow cells, mesenchymal stem cells, oxygenating materials, oxygen generating compounds, growth factors, antibiotics, anti-neoplastic agents, and combinations thereof. 
     
     
         5 . The bone repair composition of  claim 4 , wherein the oxygen generating compounds are selected from the group consisting of perchlorates, percarbonates, perborates, and peroxides, 
     
     
         6 . The bone repair composition of  claim 4 , wherein the oxygenating materials comprise a perfluorocarbon. 
     
     
         7 . The bone repair composition of  claim 6 , wherein the perfluorocarbon is perfluorodecalin, perfluorohexane, perfluorotributylamine, and/or perfluoroperhydrophenanthrene. 
     
     
         8 . The bone repair composition of  claim 1 , wherein the demineralized bone particles are dispersed within the matrix. 
     
     
         9 . The bone repair composition of  claim 1 , wherein the demineralized bone particles are in the form of a powder. 
     
     
         10 . The bone repair composition of  claim 1 , wherein the cancellous bone matrix is demineralized. 
     
     
         11 . The bone repair composition of  claim 1  wherein the bone particles are demineralized cortical bone. 
     
     
         12 . The bone repair composition of  claim 1  wherein the demineralized bone particles are a mixture of cortical and cancellous bone. 
     
     
         13 . The bone repair composition of  claim 1  wherein the demineralized bone particles are uniformly dispersed throughout the cancellous bone matrix. 
     
     
         14 . The bone repair composition of  claim 1  wherein the demineralized bone particles are non-uniformly dispersed throughout the cancellous bone matrix such that a region around the edges of the cancellous bone matrix are free of demineralized bone particles. 
     
     
         15 . The bone repair composition of  claim 1  wherein the demineralized bone particles are disc-shaped or rod-shaped, or are fibers, or combinations thereof. 
     
     
         16 . The bone repair composition of  claim 1  wherein the outer edges of the cancellous matrix are coated with a material to retain the demineralized bone particles. 
     
     
         17 . The bone repair composition of  claim 16  wherein the coating is selected from the group consisting of water soluble polymers, collagen, gelatin, and combinations thereof. 
     
     
         18 . The bone repair composition of  claim 17  wherein the water soluble polymers are selected from the group polyvinyl alcohol, polyacrylic acid, polyacrylamide, polyethylene glycol, polyethylene-propylene glycol, dextran, xanthum gum, carrageenan, carboxymethyl cellulose, hyaluronic acid, and combinations thereof. 
     
     
         19 . The bone repair composition of  claim 1 , further comprising an excipient, wherein the excipient is infused within the matrix to render it flexible. 
     
     
         20 . The bone repair composition of  claim 19 , wherein the excipient is selected from the group consisting of glycerol, lecithin, sucrose, trehalose and combinations thereof. 
     
     
         21 - 36 . (canceled)