Compositions comprising textured patterns and methods of using the same

Abstract

The invention provides method of guiding unidirectional movement of a cell comprising exposing the cell to a surface comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements for a time period sufficient to bias actin polymerization within the cell. The invention also provides methods of inducing directional movement of a cell, and methods of harnessing an actin wave within a cell. In addition, the invention provides compositions comprising a contact side, wherein at least a portion of the contact side comprises a cell contact portion comprising an asymmetric, three-dimensional pattern of one or a plurality of topographical elements, and methods of inducing healing of a wound of a subject comprising contacting the wound with the composition.

1 . A method of guiding unidirectional movement of a cell comprising exposing the cell to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the cell, wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface. 
     
     
         2 . The method of  claim 1 , wherein the topographical elements are protrusions aligned in a set of evenly spaced rows across at least a portion of the surface. 
     
     
         3 . The method of  claim 1 , wherein the three-dimensional pattern of asymmetric topographical elements comprise at least one or a plurality of saw-toothed, columnar, ridge or pyramidal shaped elements. 
     
     
         4 . The method of  claim 1 , wherein the topographical elements are saw-tooth protrusions aligned in a set of evenly spaced rows across at least a portion of the surface. 
     
     
         5 . The method of  claim 4 , wherein the saw tooth protrusions are from about 0.4 to about 2.5 microns in height, from about 0.8 to about 8.0 microns in length, from about 0.1 to about 0.8 microns in width and from about 0.8 and to about 2.2 microns in spacing between consecutive protrusions along the path or between rows. 
     
     
         6 . The method of  claim 3 , wherein the saw tooth protrusions are from about 0.1 to about 3.0 microns in height. 
     
     
         7 . The method of  claim 3 , wherein the saw tooth protrusions are from about 0.05 to about 1.00 micron in width. 
     
     
         8 . The method of  claim 3 , wherein the saw tooth protrusions are from about 0.3 to about 20.0 microns in length. 
     
     
         9 . The method of  claim 3 , wherein the saw tooth protrusions have a repeat distance from about 0.3 to about 25.0 microns. 
     
     
         10 . The method of  claim 3 , wherein the saw tooth protrusions have a pitch of from about 0.2 to about 6.0 microns. 
     
     
         11 . The method of  claim 3 , wherein the saw tooth protrusions have an offset distance from about 0 to about 12.5 microns. 
     
     
         12 . The method of  claim 1 , wherein the method comprises movement of the cell across a distance of at least about 5 microns. 
     
     
         13 . A method of inducing directional movement of a cell comprising contacting the cell to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the cell, wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface. 
     
     
         14 . The method of  claim 13  wherein the movement is across a distance of at least about 5 microns. 
     
     
         15 . A method of directing or harnessing an actin wave within a cell, the method comprising contacting the cell to a surface comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements for a time period sufficient to bias actin polymerization within the cell, wherein the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the surface. 
     
     
         16 . The method of  claim 15  wherein the movement is across a distance of at least about 5 microns. 
     
     
         17 . A composition comprising a contact side, wherein at least a portion of the contact side comprises a cell contact portion comprising a three-dimensional pattern of one or a plurality of asymmetric topographical elements, the topographical elements are from about 0.001 to about 100 microns in height or width or depth and aligned along at least one or a plurality of straight, curved or circumferential paths on the cell contact portion. 
     
     
         18 . The composition of  claim 17 , wherein the composition is a wound dressing, wherein the contact side is a subject contact side and the wound dressing comprises an oppositely facing side, wherein the subject contact side comprises: (i) an elastic, adhesive portion comprising a skin adhesive configured for adhering to a subject's skin proximate to a wound; and (ii) a wound portion comprising a non-adhesive material configured for placement over a wound, wherein the wound portion comprises the three-dimensional pattern of one or the plurality of topographical asymmetric elements; and wherein the wound dressing is free of a chemical gradient. 
     
     
         19 . The composition of  claim 17 , wherein the three-dimensional pattern of one or the plurality of asymmetric topographical elements induce directional movement of one or the plurality of cells along the at least one or the plurality of straight, curved or circumferential paths on the cell contact portion. 
     
     
         20 . The composition of  claim 17 , wherein the three-dimensional pattern comprises one or a plurality of offset, parallel paths of asymmetric saw tooth-shaped protrusions, wherein the protrusions have a repeat distance from about 0.3 to about 25.0 microns, a pitch of from about 0.2 to about 6.0 microns, and an offset distance from about 0 to about 12.5 microns. 
     
     
         21 . The composition of  claim 17 , wherein the composition is an implant configured to be implanted within in a subject, wherein the contact side is an outer surface of the implant, and wherein the implant is free of a chemical gradient. 
     
     
         22 . A method of inducing healing of a wound of a subject comprising contacting the wound with the composition of  claim 17  for a time period sufficient to bias actin polymerization within a plurality of cells at, adjacent to or proximate to the wound such that the cells unidirectionally move toward or away from a direction defined by the sidedness of the asymmetric uppermost points of the topographical elements. 
     
     
         23 . The method of  claim 22  wherein the composition comprises a surface configured for contacting a wound wherein the surface comprises a three-dimensional pattern comprising one or a plurality of offset, parallel paths of asymmetric saw tooth-shaped protrusions, wherein the protrusions have a repeat distance from about 0.3 to about 25.0 microns, a pitch of from about 0.2 to about 6.0 microns, and an offset distance from about 0 to about 12.5 microns.