Methods for cannabinoid quantification

Abstract

A method for quantification of the concentration of one or more cannabinoid compounds in a liquid sample is provided. The method involves contacting the liquid sample with at least one cannabinoid-sensitive visualization reagent, allowing the at least one cannabinoid-sensitive visualization reagent to develop for a defined amount of time; and comparing the resulting color change of the at least one cannabinoid-sensitive visualization reagent to a calibrated quantification reference chart.

1 . (canceled) 
     
     
         2 . A method for quantification of the concentration of one or more cannabinoid compounds in a liquid sample, the method comprising:
 a) contacting the liquid sample with at least one cannabinoid-sensitive visualization reagent;   b) allowing the at least one cannabinoid-sensitive visualization reagent to develop for a defined amount of time; and   c) comparing a resulting color change of the at least one cannabinoid-sensitive visualization reagent to a calibrated quantification reference chart calibrated by color to correspond to a range of color changes including the resulting color change, wherein the one or more cannabinoid compounds comprises CBD, and wherein the at least one cannabinoid-sensitive visualization reagent comprises potassium hydroxide.   
     
     
         3 . The method of  claim 2 , wherein the at least one cannabinoid-sensitive visualization reagent is present in a liquid form. 
     
     
         4 . The method of  claim 2 , wherein the at least one cannabinoid-sensitive visualization reagent is present in a solid form. 
     
     
         5 . The method of  claim 4 , wherein the at least one cannabinoid-sensitive visualization reagent is impregnated in a test strip. 
     
     
         6 . The method of  claim 5 , wherein the test strip comprises a porous matrix uniformly impregnated with the at least one cannabinoid-sensitive visualization reagent. 
     
     
         7 . A method for quantification of one or more cannabinoid compounds in a solid test sample, the method comprising:
 1) contacting the solid test sample with an extraction solvent, wherein one or more cannabinoids are extracted from the solid sample into an extraction solvent resulting in a cannabinoid-containing liquid extraction solution;   2) contacting the cannabinoid-containing liquid extraction solution with one or more cannabinoid-sensitive visualization reagents; and   3) comparing an intensity of a resulting color change of the one or more cannabinoid-sensitive visualization reagents to a calibrated quantification reference chart calibrated by color to correspond to a range of color changes including the intensity of the resulting color change, wherein the one or more cannabinoid compounds comprises CBD, and wherein the one or more cannabinoid-sensitive visualization reagents comprises potassium hydroxide.   
     
     
         8 . An apparatus comprising a test strip impregnated with at least one cannabinoid-sensitive visualization reagent, wherein the at least one more cannabinoid-sensitive visualization reagent comprises potassium hydroxide. 
     
     
         9 . A kit for quantification of the concentration of one or more cannabinoid compounds in a sample, the kit comprising:
 1) at least one cannabinoid sensitive visualization reagent; and   2) a calibrated quantification reference chart calibrated by color to correspond to a range of color changes, wherein the one or more cannabinoid compounds comprises CBD, and wherein the at least one cannabinoid-sensitive visualization reagent comprises potassium hydroxide.   
     
     
         10 . The kit of  claim 9  wherein the at least one cannabinoid-sensitive visualization reagent is present in a liquid form or a solid form. 
     
     
         11 . The kit of  claim 9 , wherein the at least one cannabinoid-sensitive visualization reagent is pre-measured as a dry reagent in one or more reaction containers. 
     
     
         12 . The kit of  claim 9 , wherein the at least one cannabinoid-sensitive visualization reagent is impregnated into a test strip.