Multilayer Compositions, Coated Devices And Use Thereof

Abstract

The present invention provides, among other things, multilayer film coating compositions, coated substrates and methods thereof. In some embodiments, a structure, comprising a substrate and a multilayer film on the substrate, wherein the multilayer film comprises a release layer and one or more layer-by-layer films. In some embodiments, a structure comprising a microneedle substrate and a multilayer film coated on at least portion of the microneedle substrate, wherein the multilayer film comprises an agent for delivery.

What is claimed is: 
     
         1 . A structure, comprising:
 a substrate, comprising a microneedle array;   a release layer disposed on the substrate; and   a controlled delivery layer disposed on the release layer, wherein the controlled delivery layer comprises one or more layer-by-layer (LBL) films.   
     
     
         2 . The structure of  claim 1 , wherein the release layer comprises a polymer, the polymer being a photocleavable polymer, convertible into a photocleaved polymer. 
     
     
         3 . The structure of  claim 1 , wherein the controlled delivery layer comprises one or more pharmaceutical agents. 
     
     
         4 . The structure of  claim 3 , wherein the one or more pharmaceutical agent is a protein, a polypeptide. 
     
     
         5 . The structure of  claim 3 , wherein the one or more pharmaceutical agent is a radionuclide, a fluorescent dye, a luminescent dye, a magnetic imaging agent, or a nutraceutical agent. 
     
     
         6 . The structure of  claim 3 , wherein the one or more pharmaceutical agent is an anti-viral agent, an anesthetic agent, an anticoagulant agent, an anti-cancer agent, a decongestant, an antihypertensive agent, a sedative agent, a birth control agent, a progestational agent, an anti-cholinergic agent, an anti-depressant agent, an anti-psychotic agent, a β-adrenergic blocking agent, a diuretic agent, a cardiovascular active agent, or a vasoactive agent. 
     
     
         7 . The structure of  claim 3 , wherein the one or more pharmaceutical agent is an antibiotic. 
     
     
         8 . The structure of  claim 7 , wherein the antibiotic is ampicillin, aziocillin, aztreonam, carbenicillin, cefoperazone, ceftriaxone, cephaloridine, cephalothin, cloxacillin, moxalactam, penicillin G, piperacillin, ticarcillin, a macrolide antibiotic, a monobactam antibiotic, a rifamycin antibiotic, a tetracycline antibiotic, a chloramphenicol antibiotic, a clindamycin antibiotic, a lincomycin antibiotic, fusidic acid, a novobiocin antibiotic, a fosfomycin antibiotic, a capreomycin antibiotic, a colistimethate antibiotic, a gramicidin antibiotic, minocycline, doxycycline, bacitracin, erythromycin, nalidixic acid, vancomycin, and trimethoprim. 
     
     
         9 . The structure of  claim 3 , wherein the pharmaceutical agent is a non-steroidal anti-inflammatory drugs (NSAIDs), a corticosteroid agent, a cycloplegic agent, or an immune selective anti-inflammatory derivative (ImSAIDs). 
     
     
         9 . The structure of  claim 2 , wherein the photocleaved polymer is pH sensitive, so that it is stable in a predetermined pH range but unstable at or near physiological pH. 
     
     
         10 . The structure of  claim 2 , wherein the photocleaved polymer is substantially less soluble at pH of 6.5 or below than at a pH of 6.5 or greater. 
     
     
         11 . The structure of  claim 2 , wherein the photocleavable polymer is a terpolymer of a hydrophobic monomer, a hydrophilic monomer, and an additional monomer having a side group represented by the following structural formula: 
       
         
           
           
               
               
           
         
       
     
     
         12 . The structure of  claim 5 , wherein the hydrophobic monomer is selected from methyl methacrylate, ethyl methacrylate, n-butyl methacrylate, n-decyl methacrylate, 2-ethylhexyl methacrylate, N-(n-octadecyl)acrylamide, n-tert-octylacrylamide, stearyl acrylate, stearyl methacrylate, and vinyl stearate. 
     
     
         13 . The structure of  claim 5 , wherein the hydrophilic monomer is selected from hydroxyethylmethacrylate, hydroxyethyl acrylate, 4-hydroxybutyl methacrylate, N-(2-hydroxypropyl)methacrylamide, n-methylmethacrylamide, acrylamide, poly(ethylene glycol) monomethyl ether methacrylates, poly(ethylene glycol) methacrylate, poly(ethylene glycol) methacrylates, and n-vinyl-2-pyrrolidone. 
     
     
         14 . The structure of  claim 5 , wherein the additional monomer is photocleavable to a carboxyl group. 
     
     
         15 . The structure of  claim 2 , wherein the photocleavable polymer is a terpolymer of methyl methacrylate, poly(ethylene glycol) methacrylate, and o-nitrobenzyl methacrylate. 
     
     
         16 . The structure of  claim 1 , wherein the LBL films comprises a first plurality of a first unit. 
     
     
         17 . The structure of  claim 10 , wherein the LBL films further comprise a second plurality of a second unit. 
     
     
         18 . The structure of  claim 1 , wherein at least a portion of the LBL films comprises alternating polycationic and polyanionic layers, and degradation of the LBL films is characterized by hydrolytic degradation of at least a portion of one of the polycationic layers, one of the polyanionic layers, or both. 
     
     
         19 . The structure of  claim 1 , wherein at least a portion of the LBL films comprises a degradable polyelectrolyte. 
     
     
         20 . The structure of  claim 13 , wherein the degradable polyelectrolyte comprises a polymer selected from polyester, polyanhydride, polyorthoester, polyphosphazene, and polyphosphoester, or any combination thereof. 
     
     
         21 . The structure of  claim 14 , wherein the degradable polyelectrolyte comprises the polyester selected from a group consisting of poly(β-amino ester)s, poly(L-lactide-co-L-lysine), poly(serine ester), poly(4-hydroxy-L-proline ester), and poly[α-(4-aminobutyl)-L-glycolic acid], or any combination thereof. 
     
     
         22 . The structure of  claim 15 , wherein the polyester is the poly(β-amino ester) selected from the group consisting of 
       
         
           
           
               
               
           
         
         wherein: 
         linker A and linker B are each independently selected from the group consisting of carbon chains of 1 to 30 carbon atoms, heteroatom-containing carbon chains of 1 to 30 atoms, and carbon chains and heteroatom-containing carbon chains with at least one substituent selected from the group consisting of branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, amino, alkylamino, dialkylamino, trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic, cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide, carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl, alkylthioether, and thiol groups; 
         R1 and R2 are each independently selected from the group consisting of hydrogen, branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, aryl, halogen, hydroxyl, alkoxy, carbamoyl, carboxyl ester, carbonyldioxyl, amide, thiohydroxyl, alkylthioether, amino, alkylamino, dialkylamino, trialkylamino, cyano, ureido, a substituted alkanoyl group, cyclic, cyclic aromatic, heterocyclic, and aromatic heterocyclic groups, each of which may be substituted with at least one substituent selected from the group consisting of branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, amino, alkylamino, dialkylamino, trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic, cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide, carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl, alkylthioether, and thiol groups; and 
         n is an integer greater than or equal to 5. 
       
     
     
         23 . The structure of  claim 15 , wherein the polyester is the poly(β-amino ester) selected from the group consisting of 
       
         
           
           
               
               
           
         
         wherein: 
         linker B is independently selected from the group consisting of carbon chains of 1 to 30 carbon atoms, heteroatom-containing carbon chains of 1 to 30 atoms, and carbon chains and heteroatom-containing carbon chains with at least one substituent selected from the group consisting of branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, amino, alkylamino, dialkylamino, trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic, cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide, carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl, alkylthioether, and thiol groups; 
         R is selected from the group consisting of hydrogen, branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, aryl, halogen, hydroxyl, alkoxy, carbamoyl, carboxyl ester, carbonyldioxyl, amide, thiohydroxyl, alkylthioether, amino, alkylamino, dialkylamino, trialkylamino, cyano, ureido, a substituted alkanoyl group, cyclic, cyclic aromatic, heterocyclic, and aromatic heterocyclic groups, each of which may be substituted with at least one substituent selected from the group consisting of branched and unbranched alkyl, branched and unbranched alkenyl, branched and unbranched alkynyl, amino, alkylamino, dialkylamino, trialkylamino, aryl, ureido, heterocyclic, aromatic heterocyclic, cyclic, aromatic cyclic, halogen, hydroxyl, alkoxy, cyano, amide, carbamoyl, carboxylic acid, ester, carbonyl, carbonyldioxyl, alkylthioether, and thiol groups; and 
         n is an integer greater than or equal to 5. 
       
     
     
         24 . The structure of  claim 15 , wherein the poly(β-amino ester) is selected from the group consisting of 
       
         
           
           
               
               
           
         
       
     
     
         25 . The structure of  claim 1 , wherein the nucleic acid comprises a therapeutic gene. 
     
     
         26 . The structure of  claim 1 , wherein the nucleic acid comprises a plasmid DNA. 
     
     
         27 . The structure of  claim 1 , wherein the nucleic acid comprises a plasmid DNA, and wherein the controlled delivery layer further includes a polymeric transfection agent and an adjuvant. 
     
     
         28 . A method of making a structure, comprising
 a substrate, comprising a microneedle array;   a release layer disposed on the substrate; and   a controlled delivery layer disposed on the release layer, wherein the controlled delivery layer comprises one or more layer-by-layer (LBL) films and at least one pharmaceutical agent,   
       the method comprising a steps of:
 coating the substrate with the release layer; and 
 coating the release layer with the controlled delivery layer. 
 
     
     
         29 . The method of  claim 22 , further comprising altering the property of the release layer to allow the release of the controlled delivery layer under certain conditions. 
     
     
         30 . The method of  claim 23 , wherein the release layer comprises a photocleavable polymer and the step of altering the property of the release layer comprises exposing the release layer to UV to obtain a photocleaved polymer. 
     
     
         31 . The method of  claim 24 , wherein the photocleaved polymer is pH sensitive, so that it is stable in a predetermined pH range but unstable at or near physiological pH. 
     
     
         32 . The method of  claim 23 , wherein the step of altering the property of the release layer is performed before the step of coating the release layer with the controlled delivery layer. 
     
     
         33 . The method of  claim 22 , further comprising incorporating at least one pharmaceutical agent into the controlled delivery layer. 
     
     
         34 . A method of delivering at least one pharmaceutical agent to a subject in need thereof, the method comprising:
 providing a structure, comprising:   a substrate, comprising a microneedle array;   a release layer disposed on the substrate; and   a controlled delivery layer disposed on the release layer, wherein the controlled delivery layer comprises one or more layer-by-layer (LBL) films and at least one pharmaceutical agent; and   contacting an application site on the subject with the structure so that the release layer releases the controlled delivery layer from the substrate into the application site.   
     
     
         35 . The method of  claim 28 , further including removing the substrate from the application site.