Topical formulation for skin care

Abstract

Disclosed herein is a method of preparing a subject's cells for topical treatment of a subject in need. Also provided is a topical formulation and methods of treating a subject suffering a skin disorder and pain using the topical formulation. Also disclosed herein are devices and systems for micronizing an aspirate in preparation of such a topical formulation.

What is claimed is: 
     
         1 . A method for micronizing an aspirate, the method comprising:
 loading an aspirate into a container;   inserting a blade into the container;   connecting the blade to a motor for causing rotation of the blade; and   causing the blade to rotate within the container to micronize the aspirate,   wherein the aspirate is micronized without use of collagenase.   
     
     
         2 . The method of  claim 1 , wherein causing the blade to rotate comprises pressing a pedal. 
     
     
         3 . The method of  claim 1 , wherein the container is a syringe barrel of a syringe. 
     
     
         4 . The method of  claim 3 , wherein loading an aspirate comprises withdrawing a plunger of the syringe. 
     
     
         5 . A method of making a cell for treatment of a subject, the method comprising:
 obtaining cells from the subject;   placing cells in growth media, wherein the growth media comprises human platelet lysate; and   growing the cells up to confluency.   
     
     
         6 . The method of  claim 5 , wherein obtaining cells from the subject comprises obtaining at least one of an aspirate, a marrow aspirate, fat aspirate or amnio/placental tissue. 
     
     
         7 . The method of  claim 6 , further comprising micronizing the aspirate or amnio/placental tissue and, wherein micronizing the aspirate or amnio/placental tissue comprises:
 loading the aspirate or amnio/placental tissue into a syringe barrel of a syringe;   inserting a blade into the syringe barrel;   connecting the blade to a handpiece, the handpiece including a motor for causing rotation of the blade; and   causing the blade to rotate within the syringe barrel to micronize the aspirate or amnio/placental tissue.   
     
     
         8 . The method of  claim 5 , wherein the growth media further comprises human serum or fibroblast differentiation media. 
     
     
         9 . The method of  claim 5 , wherein after growing the cells up to confluency, the method further comprises concentrating the cells, and after concentrating the cells, the method further comprises cryo-freezing the cells for storage. 
     
     
         10 . A method of treating a subject suffering from a skin disorder, the method comprising:
 obtaining skin cells from the skin of a subject having a skin disorder;   obtaining blood from the subject having the skin disorder;   centrifuging the blood to separate platelets and plasma;   forming platelet rich plasma by combining the separated platelets and plasma; and   providing the platelet rich plasma to the subject to treat the skin disorder.   
     
     
         11 . The method of  claim 10 , further comprising growing the skin cells to confluency in the platelet rich plasma. 
     
     
         12 . The method of  claim 10 , wherein treating the skin disorder comprises at least one of reducing spots, diminishing appearance of wrinkles, diminishing pore size, diminishing brown spots, diminishing bacteria damage, and diminishing red areas. 
     
     
         13 . A method of treating a subject suffering from a skin disorder, the method comprising:
 obtaining blood from a subject having a skin disorder;   centrifuging the blood to separate plasma and platelets;   forming platelet rich plasma by combining the separated platelets and plasma; and   providing the platelet rich plasma to the subject to treat the skin disorder,   
     
     
         14 . The method of  claim 13 , further comprising adding a base to the platelet rich plasma to form a topical formulation. 
     
     
         15 . The method of  claim 13 , wherein the providing the platelet rich plasma to the subject comprises providing a topical formulation to the subject. 
     
     
         16 . The method of  claim 13 , wherein providing the platelet rich plasma to the subject comprises providing an injectable formulation to the subject. 
     
     
         17 . The method of  claim 13 , wherein treating the skin disorder comprises reducing spots, diminishing appearance of wrinkles, diminishing pore size, diminishing brown spots, diminishing bacteria damage, diminishing red areas, reducing skin damage caused by aging, reducing skin damage caused by sun, maintaining or improving skin tone, maintaining or improving skin elasticity, or maintaining youthful appearance.