Anti-ephrinb2 antibodies and methods using same

Abstract

The invention provides anti-EphrinB2 antibodies, and compositions comprising and methods of using these antibodies.

1 . 1. An isolated anti-EphrinB2 antibody comprising:
 (a) at least one, two, three, four or five hypervariable region (HVR) sequences selected from the group consisting of:   (i) HVR-Ll comprising sequence A1-A11, wherein A1-A11 is RASQDVSTAVA (SEQ ID NO: 6)   (ii) HVR-L2 comprising sequence B1-B7, wherein B1-B7 is SASFLYS (SEQ ID NO:8)   (iii) HVR-L3 comprising sequence C1-C9, wherein C1-C9 is EQTDSTPPT (SEQ ID NO:12)   (iv) HVR-Hl comprising sequence D1-D10, wherein D1-D10 is GFTVSSGWIH (SEQ ID NO:2)   (v) HVR-H2 comprising sequence E1-E18, wherein E1-E18 is AVIFHNKGGTDY ADSVKG (SEQ ID NO:4) and   (vi) HVR-H3 comprising sequence F1-F14, wherein F1-F14 is ARTSAWAQLGAMDY (SEQ ID NO:5); and   (b) at least one variant HVR, wherein the variant HVR sequence comprises modification of at least one residue of the sequence depicted in SEQ ID NOs:1-12.   
     
     
         2 - 17 . (canceled) 
     
     
         18 . A polynucleotide encoding an antibody of  claim 1 . 
     
     
         19 . An expression vector comprising the polynucleotide of  claim 18 . 
     
     
         20 . (canceled) 
     
     
         21 . A host cell comprising an expression vector of  claim 19 . 
     
     
         22 - 24 . (canceled) 
     
     
         25 . A method for making an anti-EphrinB2 antibody, said method comprising (a) expressing a vector of  claim 19  in a suitable host cell, and (b) recovering the antibody. 
     
     
         26 . A method for making an anti-EphrinB2 immunoconjugate, said method comprising (a) expressing a vector of  claim 19  in a suitable host cell, and (b) recovering the antibody. 
     
     
         27 - 28 . (canceled) 
     
     
         29 . A method for detection of EphrinB2, the method comprising detecting a complex of comprising the anti-EphrinB2 antibody of  claim 1  and EphrinB2 in a biological sample. 
     
     
         30 . A method for diagnosing a disorder associated with EphrinB2 expression, the method comprising detecting a complex of comprising the anti-EphrinB2 antibody of  claim 1  and EphrinB2 in a biological sample from a patient having or suspected of having the disorder. 
     
     
         31 . (canceled) 
     
     
         32 . A composition comprising an anti-EphrinB2 antibody of  claim 1 . 
     
     
         33 . A composition comprising a polynucleotide of  claim 18 . 
     
     
         34 . (canceled) 
     
     
         35 . A method of inhibiting angiogenesis comprising administration of an anti-EphrinB2 antibody of  claim 1  to a subject in need of such treatment. 
     
     
         36 . The method of  claim 35 , further comprising administering to the subject an effective amount of an anti-angiogenic agent or a chemotherapeutic agent. 
     
     
         37 - 48 . (canceled)