Anti dengue activity of cissampelos pareira extracts

Abstract

The present invention relates to the anti dengue activity of the cissampelos pareira extracts. Pharmaceutical compositions comprising extracts of cissampelos pareira and processes for the preparation of extracts of cissampelos pareira are also provided.

We claim: 
     
         1 . An extract of  cissampelos pareira  for treating dengue virus infection. 
     
     
         2 . An extract of  claim 1 , which is selected from methanol, methanol:water (50:50) or aqueous extract. 
     
     
         3 . An extract of  claim 1 , which is selected from a fraction of methanol extract. 
     
     
         4 . The fraction of  claim 3 , which is selected from hexane, chloroform, dichloromethane, butanol, aqueous, ethyl acetate or acetone fraction. 
     
     
         5 . A pharmaceutical composition comprising an extract of  cissampelos pareira  along with one or more of pharmaceutically acceptable carriers, excipients or diluents for treating dengue virus infection. 
     
     
         6 . A method of treating dengue virus infection comprising administering a therapeutically effective amount of an extract of  cissampelos pareira.    
     
     
         7 . A method of treating dengue virus infection comprising administering a pharmaceutical composition comprising an extract of  cissampelos pareira  along with one or more of pharmaceutically acceptable carriers, excipients or diluents. 
     
     
         8 . Use of an extract of  cissampelos pareira  in the manufacture of a medicament for treating dengue virus infection. 
     
     
         9 . An extract of  cissampelos pareira  for treating dengue virus infection, which is prepared by a process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions, or mixture(s) thereof,   (b) concentrating the extract, and   (c) drying the extract.   
     
     
         10 . An extract of  cissampelos pareira  for treating dengue virus infection, which is prepared by a process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions or mixture(s) thereof,   (b) concentrating the extract,   (c) adding water,   (d) partitioning the extract with one or more solvents selected from water, petroleum fractions, halogenated hydrocarbons, esters, ketones, alcohols, ethers or mixture(s) thereof, and   (e) drying the extract.   
     
     
         11 . An extract of  cissampelos pareira  for treating dengue virus infection, which is prepared by a process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions or mixture(s) thereof,   (b) concentrating the extract,   (c) extracting the extract with one or more solvents selected from one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions or mixture(s) thereof, and   (d) drying the extract.   
     
     
         12 . A process for the preparation of extracts of  cissampelos pareira,  the process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions, or mixture(s) thereof,   (b) concentrating the extract, and   (c) drying the extract, wherein the extract is used for treating dengue virus infection.   
     
     
         13 . A process for the preparation of extracts of  cissampelos pareira,  the process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions, or mixture(s) thereof,   (b) concentrating the extract,   (c) adding water,   (d) partitioning the extract with one or more solvents selected from water, petroleum fractions, halogenated hydrocarbons, esters, ketones, alcohols, ethers, or mixture(s) thereof, and   (e) drying the extract, wherein the extract is used for treating dengue virus infection.   
     
     
         14 . A process for the preparation of extracts of  cissampelos pareira,  the process comprising:
 (a) extracting the plant mass of  cissampelos pareira  with one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions, or mixture(s) thereof,   (b) concentrating the extract,   (c) extracting the extract with one or more solvents selected from one or more solvents selected from water, alcohols, ketones, esters, halogenated hydrocarbons, petroleum fractions, or mixture(s) thereof, and   (d) drying the extract, wherein the extract is used for treating dengue virus infection.   
     
     
         15 . The process of  claim 9 ,  10 ,  11 ,  12 ,  13  or  14  wherein alcohol is selected from methanol, ethanol, propanol, isopropanol or butanol, ketone is selected from acetone or methyl isobutyl ketone, ester is selected from methyl acetate or ethyl acetate, halogenated hydrocarbon is selected from chloroform, dichloromethane or ethylene dichloride, ether is diethyl ether and petroleum fraction is selected from hexane, petroleum ether or heptane.