Phytophthora resistant plants belonging to the solanaceae family

Abstract

The present invention relates to a plant belonging to the Solanaceae family wherein said plant comprises a genetic trait providing Phytophthora resistance and wherein said resistance trait is encoded by a combination of at least two genes having a reduced expression, or transcription, of said genes or a reduced activity of proteins encoded by said genes as compared to said plant belonging to Solanaceae family being susceptible to Phytophthora.

1 - 11 . (canceled) 
     
     
         12 . A  Phytophthora infestans  resistant tomato plant, wherein the resistant tomato plant has a reduced activity of a first protein having amino acid sequence SEQ ID NO: 5 and a reduced activity of a second protein having amino acid sequence SEQ ID NO: 6, and wherein the activity of the first protein and the activity of the second protein are reduced in the resistant tomato plant compared to the activity of the first protein and the activity of the second protein in a tomato plant that is not resistant to  Phytophthora infestans.    
     
     
         13 . The resistant tomato plant of  claim 12 , wherein the resistant tomato plant has a non-natural mutation introduced into its genome that results in reduced expression or reduced transcription of a gene encoding the first protein and a non-natural mutation introduced into its genome that results in reduced expression or reduced transcription of a gene encoding the second protein. 
     
     
         14 . The resistant tomato plant of  claim 12 , wherein the-non-natural mutations comprise gene silencing. 
     
     
         15 . The resistant tomato plant of  claim 12 , wherein the resistant tomato plant has a non-natural mutation introduced into a gene having nucleotide sequence SEQ ID NO: 11 and a non-natural mutation introduced into a gene having nucleotide sequence SEQ ID NO: 12. 
     
     
         16 . The resistant tomato plant of  claim 15 , wherein the-non-natural mutations comprise gene silencing. 
     
     
         17 . The resistant tomato plant of  claim 16 , wherein the gene having nucleotide sequence SEQ ID NO: 11 is silenced, and the gene having nucleotide sequence SEQ ID NO: 12 is silenced. 
     
     
         18 . The resistant tomato plant of  claim 15 , wherein the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 11 reduces expression or transcription of the gene. 
     
     
         19 . The resistant tomato plant of  claim 15 , wherein the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 12 reduces expression or transcription of the gene. 
     
     
         20 . A seed, tissue, or plant part of the tomato plant of  claim 12 , wherein the seed, tissue, or plant part comprises a reduced activity of the first protein and a reduced activity of the second protein. 
     
     
         21 . The seed, tissue, or plant part of the tomato plant of  claim 20 , wherein the seed, tissue, or plant part comprises a non-natural mutation in a gene having nucleotide sequence SEQ ID NO: 11 and a non-natural mutation in a gene having nucleotide sequence SEQ ID NO: 12. 
     
     
         22 . A method for obtaining a  Phytophthora infestans  resistant tomato plant comprising:
 reducing activity of a first protein having amino acid sequence SEQ ID NO: 5 and reducing activity of a second protein having amino acid sequence SEQ ID NO: 6 in a tomato plant.   
     
     
         23 . The method of  claim 22 , wherein reducing activity of the first protein is achieved in the tomato plant by introducing a non-natural mutation into its genome that results in reduced expression or reduced transcription of a gene encoding the first protein, and wherein reducing activity of the second protein is achieved in the tomato plant by introducing a non-natural mutation into its genome that results in reduced expression or reduced transcription of a gene encoding the second protein. 
     
     
         24 . The method of  claim 23 , wherein the gene encoding the first protein has the nucleotide sequence SEQ ID NO: 11, and the gene encoding the second protein has the nucleotide sequence SEQ ID NO: 12. 
     
     
         25 . The method of  claim 23 , wherein the non-natural mutations comprise gene silencing. 
     
     
         26 . The method of  claim 25 , wherein the gene having nucleotide sequence SEQ ID NO: 11 is silenced, and the gene having nucleotide sequence SEQ ID NO: 12 is silenced. 
     
     
         27 . A  Phytophthora infestans  resistant tomato plant produced by the method of  claim 22 , wherein the tomato plant has a reduced activity of the first protein and a reduced activity of the second protein. 
     
     
         28 . A seed, tissue, or plant part of the resistant tomato plant of  claim 27 , wherein the seed, tissue, or plant part has a reduced activity of the first protein and a reduced activity of the second protein. 
     
     
         29 . A  Phytophthora infestans  resistant tomato plant produced by the method of  claim 24 , wherein the tomato plant comprises the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 11 and the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 12, and wherein the tomato plant has a reduced activity of the first protein and a reduced activity of the second protein. 
     
     
         30 . A seed, tissue, or plant part of the resistant tomato plant of  claim 29 , wherein the seed, tissue, or plant part comprises the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 11 and the non-natural mutation in the gene having nucleotide sequence SEQ ID NO: 12, and wherein the seed, tissue, or plant part has a reduced activity of the first protein and a reduced activity of the second protein.