Red blood cell storage solutions, solution additives, and methods for improving the storage of red blood cells

Abstract

Methods, additive kits and storage compositions for decreasing the deleterious effects of storage on pRBCs, for improving the aging process of stored pRBCs, and for preventing or ameliorating patient comorbidities following the transfusion or infusion of stored blood based on inhibiting acid sphingomyelinase during storage are provided.

What is claimed: 
     
         1 . A packed red blood cell (pRBC) storage solution comprising one or more acid sphingomyelinase (Asm) inhibitors. 
     
     
         2 . The pRBC storage solution according to  claim 1 , wherein the one or more Asm inhibitors is selected from Table 1. 
     
     
         3 . The pRBC storage solution according to  claim 1 , wherein the one or more Asm inhibitors comprises at least one functional Asm inhibitor. 
     
     
         4 . The pRBC storage solution according to  claim 1 , wherein the Asm inhibitor comprises Amitriptyline. 
     
     
         5 . The pRBC storage solution according to  claim 1 , wherein the Asm inhibitor comprises fluoxetine. 
     
     
         6 . The pRBC storage solution according to  claim 1 , wherein the solution comprises an AS-3 solution. 
     
     
         7 . An additive kit for formulating a pRBC storage solution comprising at least one Asm inhibitor. 
     
     
         8 . The additive kit according to  claim 7 , wherein the at least one Asm inhibitor comprises Amitriptyline. 
     
     
         9 . The additive kit according to  claim 7 , wherein the at least one Asm inhibitor comprises Fluoxetine. 
     
     
         10 . A method for preserving red blood cells, the method comprising packing the red blood cells in a storage solution comprising at least one Asm inhibitor. 
     
     
         11 . The method according to  claim 10 , wherein the Asm inhibitor comprises at least one Asm inhibitor set forth in Table 1. 
     
     
         12 . The method according to  claim 10 , wherein the Asm inhibitor comprises at least one functional inhibitor of Asm. 
     
     
         13 . The method according to  claim 10 , wherein the Asm inhibitor is Amitriptyline. 
     
     
         14 . The method according to  claim 10 , wherein the Asm inhibitor is Fluoxetine. 
     
     
         15 . A method for mitigating a complication associated with a transfusion or infusion of red blood cells, the method comprising storing the red blood cells in a storage solution comprising at least one Asm inhibitor. 
     
     
         16 . The method according to  claim 15 , wherein the complication comprises lung inflammation. 
     
     
         17 . The method according to  claim 15 , wherein the at least one Asm inhibitor is selected from the Asm inhibitors set forth in Table 1. 
     
     
         18 . The method according to  claim 15 , wherein at least one Asm inhibitor comprises a functional Asm inhibitor. 
     
     
         19 . The method according to  claim 15 , wherein the Asm inhibitor comprises Amitriptyline. 
     
     
         20 . The method according to  claim 15 , wherein the Asm inhibitor comprises Fluoxetine.