Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof

Abstract

A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.

1 - 20 . (canceled) 
     
     
         21 . An implant comprising:
 a porous body comprising collagen, wherein the body has a top surface, an opposed basal surface, and a plurality of pores of substantially uniform pore size and orientation extending from the top surface to the basal surface.   
     
     
         22 . The implant of  claim 21 , wherein about 95% of the pores have a diameter of about 200 plus or minus 100 microns. 
     
     
         23 . The implant of  claim 21 , wherein about 95% of the pores have a diameter of about 200 plus or minus 50 microns. 
     
     
         24 . The implant of  claim 21 , wherein about 98% of the pores have a diameter of about 200 plus or minus 100 microns. 
     
     
         25 . The implant of  claim 21 , wherein about 98% of the pores have a diameter of about 200 plus or minus 50 microns. 
     
     
         26 . The implant of  claim 21 , wherein the collagen comprises one selected from the group consisting of type I collagen, type II collagen, and type IV collagen. 
     
     
         27 . The implant of  claim 21 , wherein the collagen is type I collagen. 
     
     
         28 . The implant of  claim 21 , further comprising a gel disposed within the pores. 
     
     
         29 . The implant of  claim 28 , wherein the gel is a sol-gel. 
     
     
         30 . The implant of  claim 29 , wherein the sol-gel has a sol-to-gel transition temperature of about 37 degrees C. 
     
     
         31 . The implant of  claim 28 , wherein the gel comprises a plurality of chondrocytes suspended therein. 
     
     
         32 . The implant of  claim 31 , wherein the chondrocytes include human articular chondrocytes. 
     
     
         33 . The implant of  claim 28 , wherein the gel comprises a collagen solution. 
     
     
         34 . The implant of  claim 31 , wherein the porous body and the gel are in the form of a wafer-like construct having a diameter from about 4 mm to about 25 mm and a thickness from about 0.5 mm to about 5 mm. 
     
     
         35 . The implant of  claim 34 , wherein the wafer-like construct has been cut from a composition cylindrical or rectangular block structure. 
     
     
         36 . The implant of  claim 28 , wherein the gel further includes a plurality of chondrocytes suspended therein. 
     
     
         37 . The implant of  claim 36 , wherein the gel is a sol-gel that gels at a body temperature. 
     
     
         38 . The implant of  claim 36 , wherein the chondrocytes are suspended within the collagen solution at a seeding density of about 75,000-300,000 chondrocytes per 25 microliters of the collagen solution. 
     
     
         39 . The implant of  claim 34 , wherein the sol-gel is a collagen solution comprising type I collagen. 
     
     
         40 . A cartilage implant comprising:
 a porous body comprising type I collagen disposed between a top surface and an opposed basal surface of the porous body, the porous body having a plurality of pores of substantially uniform pore size and orientation extending from the top surface to the basal surface, wherein about 95% of the pores have a diameter of about 200 plus or minus 100 microns;   a sol-gel that gels at a body temperature disposed within the pores; and   a plurality of chondrocytes suspended within the sol-gel.   
     
     
         41 . The implant of  claim 40 , wherein:
 the chondrocytes include human articular chondrocytes;   the porous body and the gel are in the form of a wafer-like construct having a diameter from about 4 mm to about 25 mm and a thickness from about 0.5 mm to about 5 mm;   the sol-gel is a collagen solution comprising type I collagen; and   the chondrocytes are suspended within the collagen solution at a seeding density of about 75,000-300,000 chondrocytes per 25 microliters of the collagen solution.