Methods for Culturing Cells in an Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus

Abstract

Provided herein are models comprising a tissue-like assembly of cells tissues or organoid bodies cultured in the presence of a pulsating alternating ionic magnetic resonance field. The cells, tissues or organoid bodies are introduced into a culture unit comprising growth and nutrient modules in which the gravity vector of the growth unit is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field.

What is claimed is: 
     
         1 . A model comprising a tissue-like assembly of cultured mammalian cells or tissue produced by a method comprising the steps of:
 introducing mammalian cells or tissues into an alternating ionic magnetic resonance culture apparatus comprising:
 a nutrient module, having a proximal end and a sealable distal end, that contains a nutrient media; 
 a growth module, having a proximal end and a distal end, that contains the cells or tissue and that is filled with nutrient media and sealed to remove observable gases, said distal end of the growth module fluidly sealed to the proximal end of the nutrient module; 
 a randomizing adapter electrically connected to a randomizing mechanism and containing the nutrient module therein; and 
 an electromagnetic chamber comprising a conductive wire to produce a pulsating alternating ionic magnetic resonance field; 
   randomizing, continually, the gravity vector of the growth module with the randomzing adapter; and   generating the pulsating alternating ionic magnetic resonance field around the growth module during culturing.   
     
     
         2 . The model of  claim 1 , wherein the method to produce the model further comprises the step of modulating the alternating ionic magnetic resonance field to produce overlapping or fluctuating alternating ionic magnetic resonance frequencies at one or more modal intervals spanning about 6.5 Hz and ranging from about 7.8 Hz to about 59.9 Hz. 
     
     
         3 . The model of  claim 2 , wherein the overlapping or fluctuating alternating ionic magnetic resonance frequencies produced are about 10, 14, 15, 16, or 32 Hz. 
     
     
         4 . The model of  claim 1 , wherein the nutrient module comprises:
 a first gas-permeable membrane with a gas port disposed thereon comprising the proximal end.   
     
     
         5 . The model of  claim 1 , wherein the growth module comprises:
 a second gas-permeable membrane with a plurality of inlet/outlet ports disposed thereon comprising the proximal end; and   a baffling system and a semi-permeable membrane comprising the distal end in fluid contact with the first gas-permeable membrane.   
     
     
         6 . The model of  claim 1 , wherein the growth module and the nutrient module are disposable. 
     
     
         7 . The model of  claim 1 , wherein materials comprising the alternating ionic magnetic resonance culture apparatus are sterilizable. 
     
     
         8 . The model of  claim 1 , wherein the alternating ionic magnetic resonance field produces one or more of a modification, growth, differentiation, dedifferentiation, altered gene expression, altered transcription events or cellular regeneration via controlling the ionic transport or transcription mechanism in the cells or tissues. 
     
     
         9 . The model of  claim 1 , wherein the alternating ionic magnetic resonance field produces a change in the mammalian cellular ionic transport thereby enabling infection and an expression of mammalian viral epitopes. 
     
     
         10 . The model of  claim 1 , wherein the mammalian cells or tissues are virally or bacterially infected mammalian cells or tissues.