Srm methods in alzheimer's disease and neurological disease assays

Abstract

Provided herein are methods for developing selected reaction monitoring mass spectrometry (LC-SRM-MS) assays.

1 . A multiplexed LC-SRM-MS assay for the measurement of a plurality of proteins in a single sample comprising:
 a) generating a set of optimal peptides and corresponding transitions for each pro-tein monitored;   b) optimizing the collision energy for each transition such that interference among the transitions monitored is avoided;   c) selecting a set of transitions that have the greatest peak areas are monitored for each of the proteins, and wherein the selected transitions do not interfere with the ions in the sample;   d) monitoring the detected set of transitions for each protein in the sample, thereby measuring a plurality of proteins in the sample.   
     
     
         2 - 16 . (canceled)