Method and solution for cryopreservation of umbilical cord blood and peripheral blood

Abstract

An object of the present invention is to provide (i) a cryopreservation solution which can be used for cryopreserving umbilical cord blood, peripheral blood, and a component containing hematopoietic stem cells derived from any one of these types of blood and which has characteristics suitable for various utilization forms and (ii) use of the cryopreservation solution. In a cryopreservation method in accordance with the present invention, a cryopreservation solution containing a cryoprotectant and glucose is mixed with umbilical cord blood or peripheral blood, and a mixture thus obtained is frozen.

1 . A cryopreservation method for cryopreserving umbilical cord blood or peripheral blood, comprising:
 a mixing step of mixing a cryopreservation solution with umbilical cord blood or peripheral blood, the cryopreservation solution containing a cryoprotectant and glucose; and   a freezing step of freezing a mixture obtained in the mixing step.   
     
     
         2 . The cryopreservation method as set forth in  claim 1 , wherein the cryopreservation solution contains no thickener. 
     
     
         3 . The cryopreservation method as set forth in  claim 1 , wherein the cryopreservation solution contains no animal-derived natural component. 
     
     
         4 . The cryopreservation method as set forth in  claim 1 , wherein the cryoprotectant is dimethyl sulfoxide. 
     
     
         5 . The cryopreservation method as set forth in  claim 1 , wherein the mixture contains the cryoprotectant in an amount of 3.0 w/v % to 10.0 w/v % and the glucose in an amount of 0.25 w/v % to 5.0 w/v %. 
     
     
         6 . The cryopreservation method as set forth in  claim 1 , wherein in the mixing step, the cryopreservation solution is mixed with the umbilical cord blood or the peripheral blood in a volume ratio of 1:1 to 3:1. 
     
     
         7 . The cryopreservation method as set forth in  claim 1 , wherein the umbilical cord blood or the peripheral blood is one from which at least part of red blood cells has been removed. 
     
     
         8 . The cryopreservation method as set forth in  claim 1 , wherein the umbilical cord blood or the peripheral blood is derived from a human. 
     
     
         9 . A production method for producing frozen umbilical cord blood or frozen peripheral blood, comprising:
 a mixing step of mixing a cryopreservation solution with umbilical cord blood or peripheral blood, the cryopreservation solution containing a cryoprotectant and glucose, the umbilical cord blood or peripheral blood containing living cells; and   a freezing step of freezing a mixture obtained in the mixing step.   
     
     
         10 . Frozen umbilical cord blood or frozen peripheral blood, produced by a method recited in  claim 9 . 
     
     
         11 . A cryopreservation solution for cryopreserving umbilical cord blood or peripheral blood, comprising a cryoprotectant and glucose. 
     
     
         12 . A kit for cryopreserving umbilical cord blood or peripheral blood, comprising:
 a cryopreservation solution recited in  claim 11 ; and   a container which is cold resistant and capable of containing (i) the cryopreservation solution and (ii) umbilical cord blood or peripheral blood.