Method for producing the laminate of a sheet-shaped cell culture and fibrin gels

Abstract

A method for treating a disease associated with tissue abnormality in a subject. The method includes administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture. The laminate is produced by a method, which includes forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein: the cell culture is prepared on a culture dish and the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart. The laminate further includes a reinforcing portion made of a fibrin gel, the reinforcing portion formed by laminating the fibrin gel on the fibrin gel layer of the laminate of the cell culture and the fibrin gel.

What is claimed is: 
     
         1 . A method for treating a disease associated with tissue abnormality in a subject, comprising:
 administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture, and wherein the laminate is produced by a method comprising:   forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein:
 the cell culture is prepared on a culture dish; and 
 the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart, wherein the laminate further comprises:
 a reinforcing portion made of a fibrin gel, the reinforcing portion formed by laminating the fibrin gel on the fibrin gel layer of the laminate of the cell culture and the fibrin gel. 
 
   
     
     
         2 . The method according to  claim 1 , wherein the reinforcing portion is made on an end of the laminate. 
     
     
         3 . The method according to  claim 1 , further comprising:
 after forming the fibrin gel layer, trimming excess fibrin gel.   
     
     
         4 . The method according to  claim 1 , comprising:
 suturing the laminate of the fibrin gel and the cell culture to the tissue abnormality in the subject.   
     
     
         5 . The method according to  claim 1 , comprising:
 a ratio of fibrinogen to thrombin adhering to the laminate of about 8:5 to about 8:75.   
     
     
         6 . A method for treating a disease associated with tissue abnormality in a subject, comprising:
 administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture, and wherein the laminate is produced by a method comprising:
 forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein:
 the cell culture is prepared on a culture dish; 
 the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart; and 
 
 suturing the laminate of the fibrin gel and the cell culture to the tissue abnormality in the subject. 
   
     
     
         7 . The method according to  claim 6 , wherein the laminate further comprises:
 a reinforcing portion made of a fibrin gel, the reinforcing portion formed by laminating the fibrin gel on the fibrin gel layer of the laminate of the cell culture and the fibrin gel.   
     
     
         8 . The method according to  claim 7 , wherein the reinforcing portion is made on an end of the laminate. 
     
     
         9 . The method according to  claim 6 , further comprising:
 after forming the fibrin gel layer, trimming excess fibrin gel.   
     
     
         10 . The method according to  claim 6 , comprising:
 suturing the reinforcing portion of the laminate of the fibrin gel and the cell culture to the tissue abnormality in the subject.   
     
     
         11 . The method according to  claim 6 , comprising:
 a ratio of fibrinogen to thrombin adhering to the laminate of about 8:5 to about 8:75.   
     
     
         12 . A method for treating a disease associated with tissue abnormality in a subject, comprising:
 administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture, and wherein the laminate is produced by a method comprising:
 forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein:
 the cell culture is prepared on a culture dish; and 
 the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart; and 
 
 placing a side on which the fibrin gel is not formed of the laminate of the fibrin gel and the cell culture on a surface of a subject's heart. 
   
     
     
         13 . The method according to  claim 12 , wherein the side on which the fibrin gel is not formed of the laminate had been attached to a surface of culture dish. 
     
     
         14 . The method according to  claim 12 , wherein the laminate further comprises:
 a reinforcing portion made of a fibrin gel, the reinforcing portion formed by laminating the fibrin gel on the fibrin gel layer of the laminate of the cell culture and the fibrin gel.   
     
     
         15 . The method according to  claim 14 , wherein the reinforcing portion is made on an end of the laminate. 
     
     
         16 . The method according to  claim 12 , further comprising:
 after forming the fibrin gel layer, trimming excess fibrin gel.   
     
     
         17 . The method according to  claim 15 , comprising:
 suturing the reinforcing portion of the laminate of the fibrin gel and the cell culture to the tissue abnormality in the subject.   
     
     
         18 . The method according to  claim 12 , comprising:
 a ratio of fibrinogen to thrombin adhering to the laminate of about 8:5 to about 8:75.