CORN EVENT PV-ZMGT32(nk603) AND COMPOSITIONS AND METHODS FOR DETECTION THEREOF

Abstract

The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.

1 . A DNA construct comprising: a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter; (ii) a rice actin 1 intron; (iii) a chloroplast transit peptide encoding DNA molecule; (iv) a glyphosate tolerant EPSPS encoding DNA molecule; and (v) a transcriptional terminator DNA molecule; and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter; (b) a Hsp70 intron; (c) a chloroplast transit peptide encoding DNA molecule; (d) a glyphosate tolerant EPSPS encoding DNA molecule; and (e) a transcriptional terminator DNA molecule. 
     
     
         2 . A DNA construct of  claim 1 , wherein the glyphosate tolerant EPSPS encoding DNA molecule consists of an AGRTU.aroA:CP4 DNA molecule. 
     
     
         3 . A plant comprising the DNA construct of  claim 2 . 
     
     
         4 . A plant of  claim 3 , wherein said plant is a corn plant. 
     
     
         5 . A corn plant comprising a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12. 
     
     
         6 . A DNA molecule comprising the construct of  claim 1  and a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12. 
     
     
         7 - 11 . (canceled) 
     
     
         12 . A method of detecting the presence of a DNA molecule selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8 in a DNA sample, the method comprising:
 (a) obtaining a DNA sample from a corn plant;   (b) contacting the DNA sample with a DNA molecule comprising a nucleotide sequence comprising sufficient length of contiguous nucleotides of SEQ ID NO:7, SEQ ID NO:8, or full complement thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12, and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12;   (c) subjecting the sample and probe to stringent hybridization conditions; and detecting hybridization of the probe to the DNA molecule in the sample.   
     
     
         13 - 16 . (canceled) 
     
     
         17 . A DNA molecule comprising a nucleotide sequence comprising a sufficient length of contiguous nucleotides of SEQ ID NO:7, SEQ ID NO:8, or full complement thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12, and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs:7-12.