Nutritional compositions for promoting gut barrier function and ameliorating visceral pain

Abstract

The present disclosure is directed to methods for i) promoting gut regeneration, ii) promoting gut maturation and/or adaptation, iii) supporting gut barrier resistance and/or iv) protecting gut barrier function in a pediatric subject comprising administering to the pediatric subject a composition comprising an effective amount of a soluble mediator preparation derived from a late-exponential growth phase of a probiotic batch-cultivation process, such as Lactobacillus rhamnosus GG (LGG). The present methods advantageously provide the gut-protection benefits of LGG soluble mediators without introducing live bacterial culture to individuals with impaired gut barrier function. In some embodiments, the pediatric subject has impaired gut barrier function and/or short bowel syndrome. The present disclosure in other embodiments, directed methods for reducing visceral pain in a pediatric subject comprising administering to the pediatric subject a composition comprising an effective amount of a soluble mediator preparation from a late-exponential growth phase of a probiotic batch-cultivation process, such as LGG.

1 - 14 . (canceled) 
     
     
         15 . An infant formula for i) promoting gut barrier regeneration, ii) promoting gut barrier maturation, iii) supporting gut barrier resistance and/or iv) protecting gut barrier function in an infant comprising:
 up to about 7 g/100 Kcal of a fat or lipid;   up to about 5 g/100 Kcal of a protein source or a protein equivalent source;   up to about 22 g/100 kcal of a carbohydrate source; and   a soluble mediator preparation from a late-exponential growth phase of a  Lactobacillus rhamnosus  GG (LGG) batch-cultivation process.   
     
     
         16 - 19 . (canceled) 
     
     
         20 . A method for reducing visceral pain sensitivity in a pediatric subject in need thereof, comprising:
 administering to the pediatric subject a composition comprising an effective amount of a soluble mediator preparation from a late-exponential growth phase of a probiotic batch-cultivation process.   
     
     
         21 - 30 . (canceled) 
     
     
         31 . The infant formula of  claim 15 , wherein the soluble mediator preparation is produced by (a) subjecting LGG to cultivation in a suitable culture medium using a batch process; (b) harvesting a culture supernatant at a late exponential growth phase of the cultivation step, which phase is defined with reference to the second half of the time between the lag phase and the stationary phase of the batch-cultivation process; (c) optionally removing low molecular weight constituents from the supernatant so as to retain molecular weight constituents above 5 or 6 kDa; (d) removing any remaining cells by 0.2 μm sterile filtration to provide the soluble mediator preparation; (e) removing liquid contents from the soluble mediator preparation so as to obtain the composition. 
     
     
         32 . The infant formula of  claim 31 , wherein step (b) further comprises removal of bacterial cells by sterile filtration. 
     
     
         33 . The infant formula of  claim 31 , wherein the late exponential phase is defined with reference to the latter quarter portion of the time between the lag phase and the stationary phase of the batch-cultivation process. 
     
     
         34 . The infant formula of  claim 31 , wherein the batch cultivation is conducted in a culture medium devoid of polysorbates. 
     
     
         35 . The infant formula of  claim 34 , wherein the medium contains an ingredient selected from the group consisting of oleic acid, linseed oil, olive oil, rape seed oil, sunflower oil, and mixtures thereof. 
     
     
         36 . The infant formula of  claim 31 , wherein the batch cultivation is conducted at a pH of from 5-7. 
     
     
         37 . The infant formula of  claim 31 , which further comprises about 0.1 g/100 kcal to about 1 g/100 kcal of a prebiotic composition, wherein the prebiotic composition comprises polydextrose, galactooligosaccharide or a combination thereof. 
     
     
         38 . The infant formula of  claim 37 , wherein polydextrose and galactooligosaccharides comprise at least about 20% of the prebiotic composition. 
     
     
         39 . The infant formula of  claim 31 , further comprising arachidonic acid, wherein the ARA and DHA are present in a weight ratio ranging from about 1:3 to 9:1. 
     
     
         40 . The infant formula of  claim 31 , further comprising lactoferrin. 
     
     
         41 . The method of  claim 20 , wherein the soluble mediator preparation is produced by (a) subjecting LGG to cultivation in a suitable culture medium using a batch process; (b) harvesting a culture supernatant at a late exponential growth phase of the cultivation step, which phase is defined with reference to the second half of the time between the lag phase and the stationary phase of the batch-cultivation process; (c) optionally removing low molecular weight constituents from the supernatant so as to retain molecular weight constituents above 5 or 6 kDa; (d) removing any remaining cells by 0.2 μm sterile filtration to provide the soluble mediator preparation; (e) removing liquid contents from the soluble mediator preparation so as to obtain the composition. 
     
     
         42 . The method of  claim 41 , wherein step (b) further comprises removal of bacterial cells by sterile filtration. 
     
     
         43 . The method of  claim 41 , wherein the late exponential phase is defined with reference to the latter quarter portion of the time between the lag phase and the stationary phase of the batch-cultivation process. 
     
     
         44 . The method of  claim 20 , wherein the batch cultivation is conducted in a culture medium devoid of polysorbates. 
     
     
         45 . The method of  claim 44 , wherein the medium contains an ingredient selected from the group consisting of oleic acid, linseed oil, olive oil, rape seed oil, sunflower oil, and mixtures thereof. 
     
     
         46 . The method of  claim 20 , wherein the batch cultivation is conducted at a pH of from 5-7. 
     
     
         47 . The method of  claim 20 , wherein the effective amount is equivalent to about 1×10 4  to about 1×10 12  cell equivalents of live probiotic bacteria per kg body weight per day.