Plasma autoantibody biomarkers for basal like breast cancer

Abstract

Cancer patients make antibodies to tumor-derived proteins that are potential biomarkers for early detection. Twenty-eight antigens have been identified as potential biomarkers for the early detection of basal-like breast cancer (Tables 1, 2). Also, a 13-AAb classifier has been developed that differentiate patients with BLBC from healthy controls with 33% sensitivity at 98% specificity (Table 3).

What is claimed is: 
     
         1 . A method for detection of basal-like breast cancer in a patient sample harboring one or more antigens, comprising:
 detecting a tumor antigen biomarker in said patient sample, wherein the biomarker is selected from the antigens: P53 (TP53), NY-ESO-1 (CTAG1B), NY-ESO-2 (CTAG2), RNF216, PPHLN1, PIP4K2C, ZBTB16, TAS2R8, WBP2NL, DOK2, PSRC1, MN1, TRIM21, POU4F1, SSMEM1, LMO4, BCL2, KRT8, TSGA13, PVRL4, SNRK, DYRK3, RNF32, JUNB, KCNIP3, CCDC68, CSN3, TRAIP.   
     
     
         2 . The method of  claim 1 , wherein said detecting comprises utilizing an enzyme-linked immunosorbant assay (ELISA). 
     
     
         3 . The method of  claim 1 , further comprising detecting at least two of said tumor antigen biomarkers and comparing a level of detection to predetermined sensitivity and specificity threshold to determine if basal-like breast cancer is indicated. 
     
     
         4 . A basal-like breast cancer diagnostic test kit, comprising an antibody against a tumor antigen biomarker selected from the group consisting of: P53 (TP53), NY-ESO-1 (CTAG1B), NY-ESO-2 (CTAG2), RNF216, PPHLN1, PIP4K2C, ZBTB16, TAS2R8, WBP2NL, DOK2, PSRC1, MN1, TRIM21, POU4F1, SSMEM1, LMO4, BCL2, KRT8, TSGA13, PVRL4, SNRK, DYRK3, RNF32, JUNB, KCNIP3, CCDC68, CSN3, TRAIP, and a suitable detection agent. 
     
     
         5 . The kit of  claim 4 , wherein an antibody against each of said tumor antigen biomarkers is present in said kit. 
     
     
         6 . A method for diagnosing basal-like breast cancer from a patient sample harboring one or more antigens, comprising:
 a) detecting a tumor antigen biomarker in said patient sample, wherein the biomarker is selected from the antigens: P53 (TP53), NY-ESO-1 (CTAG1B), NY-ESO-2 (CTAG2), RNF216, PPHLN1, PIP4K2C, ZBTB16, TAS2R8, WBP2NL, DOK2, PSRC1, MN1, TRIM21, POU4F1, SSMEM1, LMO4, BCL2, KRT8, TSGA13, PVRL4, SNRK, DYRK3, RNF32, JUNB, KCNIP3, CCDC68, CSN3, TRAIP; and   b) comparing a detection result of step a) with a control.