Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase

Abstract

The present invention relates to the use of tissue non-specific alkaline phosphatase (TNAP) as a marker for identifying and/or isolating adult multipotential cells. The present invention also relates to cell populations enriched by methods of the present invention and therapeutic uses of these cells.

1 - 45 . (canceled) 
     
     
         46 . An enriched population of TNAP +  adult multipotential cells, wherein at least 4% of the total cell population are TNAP + , STRO-1 +  multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes, wherein the TNAP +  marker is a TNAP +  marker which binds the STRO-3 antibody produced by the hybridoma cell line deposited under ATCC Accession Number PTA-5582. 
     
     
         47 . An enriched population according to  claim 46 , wherein at least 10% of the total cell population are TNAP + , STRO-1 +  multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes. 
     
     
         48 . An enriched population according to  claim 46 , wherein at least 20% of the total cell population are TNAP + , STRO-1 +  multipotential cells capable of being cultured in vitro to produce adipocytes, osteocytes and chondrocytes. 
     
     
         49 . An enriched population according to  claim 46 , wherein the STRO-1 +  cells are STRO-1 bright . 
     
     
         50 . An enriched population according to  claim 47 , wherein the STRO-1 +  cells are STRO-1 bright . 
     
     
         51 . An enriched population according to  claim 48 , wherein the STRO-1 +  cells are STRO-1 bright . 
     
     
         52 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to  claim 46  in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type. 
     
     
         53 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to  claim 47  in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type. 
     
     
         54 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to  claim 48  in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type. 
     
     
         55 . A method of generating a committed cell population of a specific tissue type, the method comprising culturing a population of adult multipotential cells according to  claim 49  in the presence of one or more stimulatory factors; and subjecting said cultured population to conditions biasing differentiation of the adult muitipotential cells to the specific tissue type. 
     
     
         56 . A composition comprising the enriched population of adult multipotential cells according to  claim 46 . 
     
     
         57 . A composition comprising the enriched population of adult multipotential cells according to  claim 47 . 
     
     
         58 . A composition comprising the enriched population of adult multipotential cells according to  claim 48 . 
     
     
         59 . A composition comprising the enriched population of adult multipotential cells according to  claim 49 . 
     
     
         60 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to  claim 46  so as to generate or repair the tissue. 
     
     
         61 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to  claim 47  so as to generate or repair the tissue. 
     
     
         62 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to  claim 48  so as to generate or repair the tissue. 
     
     
         63 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the enriched cell population according to  claim 49  so as to generate or repair the tissue. 
     
     
         64 . A method for generating or repairing tissue in a subject in need thereof, the method comprising administering to the subject the composition according to  claim 56  so as to generate or repair the tissue.