Kits for determining male fertility by measuring levels of a2v-atpase, g-csf, mip 1 alpha, mcp-1, and methods and kits for improving reproductive outcomes in artificial insemination procedures

Abstract

The present invention provides a kit for determining male fertility including a container system containing a plurality of enzyme-linked antibodies one of each capable of binding to analytes Atp6v0a2, G-CSF, MIP 1α, and MCP-1. The kit further includes suitable packaging and a set of instructions for using the enzyme-linked antibodies with a seminal sample to determine the fertility of the sample.

1 - 18 . (canceled) 
     
     
         19 . A kit for determining male fertility comprising:
 a first container of a substrate-and-antibody structure, the antibody being specific for the antigen of SEQ ID NO. 1,   packaging for holding the first container; and   instructions for contacting the substrate-and-antibody structure with a seminal sample to determine the fertility of the sample.   
     
     
         20 . The kit of  claim 19  wherein the seminal sample is sperm free. 
     
     
         21 . The kit of  claim 19  further comprising a second container of a detection antibody specific for the antigen of SEQ ID NO. 1. 
     
     
         22 . The kit of  claim 21  further comprising a third container of an enzyme-linked antibody. 
     
     
         23 . The kit of  claim 22  further comprising a fourth container of a substrate for the enzyme when converted by the enzyme provides a perceptible signal. 
     
     
         24 . The kit of  claim 23  wherein the substrate is selected from the group consisting of a microsphere and a surface of a microtiter plate. 
     
     
         25 . The kit of  claim 24  wherein the microsphere is made from plastic or metal. 
     
     
         26 . The kit of  claim 24  wherein the substrate is a microtiter plate surface coated with a plurality of capture antibodies.