US2018080032A1PendingUtilityA1

Biosynthetic pathways and methods

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Assignee: UNIV MINNESOTAPriority: Dec 18, 2012Filed: Dec 4, 2017Published: Mar 22, 2018
Est. expiryDec 18, 2032(~6.4 yrs left)· nominal 20-yr term from priority
C12Y 401/01007C12Y 102/01026C12Y 301/01068C12Y 402/01082C12Y 101/01C12Y 101/01001C12N 9/0006C12Y 301/01C12Y 402/00C12N 9/18C12N 9/88C12N 15/52C12P 7/18C12N 9/0008
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Claims

Abstract

This disclosure describes a recombinant microbial cells and methods of making and using such recombinant microbial cells. Generally, the recombinant cells may be modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. In some embodiments, the TCA derivative can include 1,4-butanediol. In various embodiments, the microbial cell is a fungal cell or a bacterial cell. In some embodiments, the increased biosynthesis of the TCA derivative can include an increase in xylose dehydrogenase activity, xylonolactonase activity, xylonate dehydratase activity, or 2-keto-3-deoxyaldonic acid dehydratase activity.

Claims

exact text as granted — not AI-modified
1 . A recombinant microbial cell modified to exhibit increased biosynthesis of a TCA derivative compared to a wild-type control. 
     
     
         2 - 134 . (canceled)

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