Systems and methods for isolating nucleic acids

Abstract

The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating nucleic acids from aqueous samples (e.g., blood or urine).

1 - 13 . (canceled) 
     
     
         14 . A kit, comprising:
 a) a detergent;   b) a bead-beating bead;   c) a proteinase;   d) isopropanol;   d) a lysis solution;   e) a wash buffer comprising isopropanol; and   f) a solid support.   
     
     
         15 . The kit of  claim 14 , wherein said bead-beating bead is a Zirconnia/Yttria bead. 
     
     
         16 . The kit of  claim 14 , wherein said proteinase is proteinase K. 
     
     
         17 . The kit of  claim 14 , wherein said solid support is a bead. 
     
     
         18 . The kit of  claim 17 , wherein said bead is a magnetic particle. 
     
     
         19 . The kit of  claim 14 , further comprising control DNA and/or RNA. 
     
     
         20 . The kit of  claim 14 , further comprising carrier RNA/Poly A. 
     
     
         21 . The kit of  claim 14 , further comprising a primer. 
     
     
         22 . The kit of  claim 21 , wherein said primer is one primer of an amplification primer pair. 
     
     
         23 . The kit of  claim 14 , further comprising an amplification enzyme. 
     
     
         24 . The kit of  claim 14 , further comprising a probe. 
     
     
         25 . The kit of  claim 24 , wherein said probe is a labeled probe. 
     
     
         26 . The kit of  claim 24 , wherein said probe comprises a stem-loop structure. 
     
     
         27 . The kit of  claim 14 , further comprising a reverse transcriptase enzyme.