Templated nanoconjugates

Abstract

The present disclosure is directed to compositions comprising templated nanoconjugates and methods of their use.

1 . A plurality of nanoconjugates, each nanoconjugate having a defined structure and comprising a plurality of crosslinked biomolecules in a monolayer, said structure defined by a surface providing a template upon which the structure is assembled, wherein the surface is optionally at least partially removed after the structure has been defined. 
     
     
         2 . The composition of  claim 1  wherein the surface is a nanoparticle. 
     
     
         3 - 6 . (canceled) 
     
     
         7 . The composition of  claim 1  wherein each biomolecule in the plurality of biomolecules is the same. 
     
     
         8 - 16 . (canceled) 
     
     
         17 . The composition of  claim 1  wherein the plurality of nanoconjugates further comprises an additional agent selected from the group consisting of a polynucleotide, peptide, polypeptide, phospholipid, oligosaccharide, metal complex, small molecule, therapeutic agent, contrast agent and combinations thereof. 
     
     
         18 - 23 . (canceled) 
     
     
         24 . The composition of  claim 1  wherein at least one nanoconjugate in the plurality of nanoconjugates is hollow in the absence of the surface. 
     
     
         25 - 26 . (canceled) 
     
     
         27 . The composition of  claim 24  wherein an additional agent is encapsulated in the nanoconjugate which is otherwise hollow. 
     
     
         28 - 34 . (canceled) 
     
     
         35 . The method of claim  28  wherein the surface is at least partially removed after the crosslinking. 
     
     
         36 . (canceled) 
     
     
         37 . The method of claim  28  wherein the first biomolecule and the second biomolecule comprise at least one alkyne moiety, or wherein the first biomolecule and the second biomolecule each comprise about 10 alkyne moieties. 
     
     
         38 - 42 . (canceled) 
     
     
         43 . The method of claim  28  wherein the first biomolecule and/or the second biomolecule is a polynucleotide. 
     
     
         44 - 46 . (canceled) 
     
     
         47 . The method of claim  28  wherein an additional agent is added to the first biomolecule and the second biomolecule during the activating step, or wherein an additional agent is added to the nanoconjugate after formation of the nanoconjugate but before the at least partial removal of the surface or wherein an additional agent is added to the nanoconjugate after formation of the nanoconjugate and after the at least partial removal of the surface. 
     
     
         48 - 49 . (canceled) 
     
     
         50 . A composition comprising a polyvalent nanoconjugate comprising a surface, the nanoconjugate further comprising a plurality of polynucleotides wherein a spacer end of each of the polynucleotides in the plurality is modified such that contacting the plurality of polynucleotides with a chemical crosslinks the plurality of polynucleotides, wherein the surface is optionally at least partially removed after the crosslinking. 
     
     
         51 . The composition of  claim 50  wherein the modification is selected from the group consisting of an amine, amide, alcohol, ester, aldehyde, ketone, thiol, disulfide, carboxylic acid, phenol, imidazole, hydrazine, hydrazone, azide and an alkyne. 
     
     
         52 - 54 . (canceled) 
     
     
         55 . The composition of  claim 50  wherein the surface comprises a nanoparticle. 
     
     
         56 - 65 . (canceled) 
     
     
         66 . A method of inhibiting expression of a gene product encoded by a target polynucleotide comprising contacting the target polynucleotide with a composition of  claim 1  under conditions sufficient to inhibit expression of the gene product. 
     
     
         67 . The method of  claim 66  wherein expression of the gene product is inhibited in vivo or in vitro. 
     
     
         68 - 69 . (canceled)