Method for producing megakaryocytes, platelets and/or thrombopoietin using mesenchymal cells

Abstract

Provided is a megakaryocyte and/or platelet production method, enabling to produce a megakaryocyte and/or platelet from mesenchymal cells such as preadipocytes in a relatively short period of time, simply, in a large amount and at lower cost or more efficiently in vitro and a method for producing TPO simply and in a larger amount. A first invention is a method for producing a megakaryocyte and/or platelet, comprising culturing a mesenchymal cell in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting megakaryocytes and/or platelets from a culture. A second invention is a method for producing thrombopoietin, comprising culturing a mesenchymal cell or mesenchymal cell-derived megakaryocyte in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter and collecting thrombopoietin from a culture. A third invention is a method for producing thrombopoietin, comprising culturing a preadipocyte in a preadipocyte culturing basic medium containing dexamethasone, 3-isobutyl-1-methylxanthine and insulin and collecting thrombopoietin from a culture.

1 - 18 . (canceled) 
     
     
         19 . A method for producing thrombopoietin, comprising culturing a mesenchymal cell or a mesenchymal cell-derived megakaryocyte in a mesenchymal cell culturing basic medium containing an iron ion and an iron transporter, and collecting thrombopoietin from a culture. 
     
     
         20 . A method for producing thrombopoietin, comprising culturing a preadipocyte in a preadipocyte culturing basic medium containing an agent for inducing differentiation into a mature adipocyte and collecting thrombopoietin from a culture. 
     
     
         21 . The method for producing thrombopoietin according to  claim 20 , wherein the agent for inducing differentiation into a mature adipocyte is one or more selected from the group consisting of 3-isobutyl-1-methylxanthine, insulin, dexamethasone and indomethacin. 
     
     
         22 . The production method according to  claim 19 , wherein the mesenchymal cell is a CD31 negative and CD71 positive mesenchymal cell. 
     
     
         23 . The production method according to  claim 19 , wherein the iron transporter is transferrin. 
     
     
         24 . The production method according to  claim 19 , wherein the iron ion and iron transporter are an iron-bound transferrin. 
     
     
         25 . The production method according to  claim 19 , wherein the iron ion and iron transporter are an iron-bound transferrin. 
     
     
         26 . The production method according to  claim 19 , wherein the culturing is performed for 4 to 7 days. 
     
     
         27 . The production method according to  claim 19 , wherein a yield of TPO per 1 mL of medium is 30 pg to 100 pg. 
     
     
         28 . The production method according to  claim 19 , further comprising a step of purifying TPO from the culture. 
     
     
         29 . The production method according to  claim 19 , wherein the medium does not contain exogenous thrombopoietin.