Gene for controlling erectness growth of rice leaf blades and application thereof

Abstract

A gene for controlling the building of architecture of gramineous crops and application thereof. The gene CYC U4;1 (Os10g41430) has a specific expression in a rice pulvinus (which is believed to have a specific expression in other gramineous crops), with a CDS sequence as shown in SEQ ID NO: 2, and an encoded amino acid sequence as shown in SEQ ID NO: 3. Also a promoter sequence of the gene, with the sequence as shown in SEQ ID NO: 1. Transgenic lines obtained by cloning a promoter and full-length CDS of CYC U4;1 to pCAMBIA1301 and transferring this into rice Nipponbare are all characterized by having smaller leaf-stem angles than those of wild rice, accordingly, the expression level of the gene CYC U4;1 can be increased or decreased with genetic engineering technology to control the plant architecture development, thereby improving the plant architecture and increasing the density of germplasm.

We claim: 
     
         1 . A protein having SEQ ID NO: 3. 
     
     
         2 . A gene encoding the protein as described in  claim 1 . 
     
     
         3 . The gene according to  claim 2 , having SEQ ID NO: 2. 
     
     
         4 . A promoter of the gene as described in  claim 2 , wherein the promoter has a sequence as shown in SEQ ID NO: 1. 
     
     
         5 . A promoter of the gene as described in  claim 3 , wherein the promoter has a sequence as shown in SEQ ID NO: 1. 
     
     
         6 . A method for controlling leaf erectness development of rice plants according to  claim 1 , comprising
 applying the protein as described in  claim 1  in a rice plant.   
     
     
         7 . A method for controlling leaf erectness development of rice plants according to  claim 2 , comprising
 expressing a gene as described in  claim 2  in a rice plant.   
     
     
         8 . A method for improving architecture of rice varieties and increasing planting density per unit area according to  claim 1 , comprising
 applying the protein as described in  claim 1  in a rice plant.   
     
     
         9 . A method for improving architecture of rice varieties and increasing planting density per unit area according to  claim 2 , comprising
 expressing a gene as described in  claim 2  in a rice plant.   
     
     
         10 . A method for decreasing leaf-stem angles of rice according to  claim 1 , comprising
 applying the protein as described in  claim 1  in a rice plant.   
     
     
         11 . A method for decreasing leaf-stem angles of rice according to  claim 3 , comprising
 expressing a gene as described in  claim 3  in a rice plant.   
     
     
         12 . A method for decreasing leaf-stem angles of rice according to  claim 4 , comprising
 applying the promoter as described in  claim 4  of the gene in a rice plant.   
     
     
         13 . A method for improving architecture of graminaceous crops according to  claim 1 , comprising
 applying the protein as described in  claim 1  in a rice plant.   
     
     
         14 . A method for improving architecture of graminaceous crops according to  claim 3 , comprising
 expressing a gene as described in  claim 3  in a rice plant.   
     
     
         15 . A method for improving architecture of graminaceous crops according to  claim 4 , comprising
 applying the promoter as described in  claim 4  of the gene in a rice plant.   
     
     
         16 . A gene having a nucleotide sequence that is at least 50% homology with the nucleotide sequence as shown in SEQ ID NO: 2. 
     
     
         17 . A protein having an amino acid sequence that is at least 50% homology with the amino acid sequence as shown in SEQ ID NO: 3.