US2018195037A1PendingUtilityA1

Three-Dimensional Fibrous Scaffolds for Cell Culture

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Assignee: MOHAPATRA SUBHRAPriority: Feb 23, 2012Filed: Apr 18, 2017Published: Jul 12, 2018
Est. expiryFeb 23, 2032(~5.6 yrs left)· nominal 20-yr term from priority
Inventors:Subhra MohapatraShyam S. MohapatraYvonne Kathleen DavisChunyan Wang
C12N 2533/72C12N 2533/40C12N 5/0068C12N 5/0693C12N 5/0062
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Claims

Abstract

Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein.

Claims

exact text as granted — not AI-modified
1 . A three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). 
     
     
         2 . The composition of  claim 1 , wherein the ratio of PEG-PLA to PLGA is approximately 1:4. 
     
     
         3 . The composition of  claim 1 , wherein the ratio of PEG-PLA to PLGA is approximately 1:10. 
     
     
         4 . The composition of  claim 1 , wherein fiber diameter ranges from approximately 0.69 to 4.18 μm. 
     
     
         5 . The composition of  claim 1 , wherein the scaffold comprises pores having a diameter of less than approximately 10 μm. 
     
     
         6 . The composition of  claim 1 , wherein the PEG has a molecular weight of approximately 2 kDa. 
     
     
         7 . The composition of  claim 1 , wherein the PLGA has a lactic acid:glycolic acid ratio of approximately 85:15. 
     
     
         8 . The composition of  claim 1 , wherein the PEG has a molecular weight of approximately 2 kDa, and wherein the PLGA has a lactic acid:glycolic acid ratio of approximately 85:15. 
     
     
         9 . The composition of  claim 1 , wherein the fibers consist essentially of a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). 
     
     
         10 . The composition of  claim 9 , wherein the ratio of PEG-PLA to PLGA is approximately 1:4. 
     
     
         11 . The composition of  claim 9 , wherein the ratio of PEG-PLA to PLGA is approximately 1:10. 
     
     
         12 . The composition of  claim 9 , wherein the PEG has a molecular weight of approximately 2 kDa. 
     
     
         13 . The composition of  claim 9 , wherein the PLGA has a lactic acid:glycolic acid ratio of approximately 85:15. 
     
     
         14 . The composition of  claim 9 , wherein the PEG has a molecular weight of approximately 2 kDa, and wherein the PLGA has a lactic acid:glycolic acid ratio of approximately 85:15. 
     
     
         15 . The composition of  claim 1 , further comprising a cell. 
     
     
         16 . The composition of  claim 15 , wherein the cell is a cancer cell. 
     
     
         17 . The composition of  claim 1 , further comprising a cancer cell spheroid. 
     
     
         18 . The composition of  claim 1 , further comprising a chitosan coating.

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