Cell tissue gel containing collagen and hyaluronan

Abstract

Described herein is a cell tissue gel containing collagen and hyaluronan at a weight ratio of 0.01-100:1.

What is claimed is: 
     
         1 . A cell tissue gel, comprising collagen and hyaluronan at a weight ratio of 1-50:1, wherein the cell tissue gel promotes wound healing with reduced scarring and is foamed without a cross-linking agent. 
     
     
         2 . The cell tissue gel of  claim 1 , wherein the concentration of collagen is 1 to 100 mg/ml and the concentration of hyaluronan is 0.5 to 100 mg/ml. 
     
     
         3 . The cell tissue gel of  claim 1 , further comprising a nutrient or a bioactive agent. 
     
     
         4 . The cell tissue gel of  claim 3 , wherein the bioactive agent is a growth factor selected from the group consisting of epidermal growth factor, fibroblast growth factor, vascular endothelial growth factor, connective tissue growth factor, platelet-derived growth factor, insulin-like growth factor, nerve growth factor, hepatocyte growth factor, colony-stimulating factor, stem cell factor, keratinocyte growth factor, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, glial derived neurotrophic factor, ciliary neurotrophic factor, endothelial-monocyte activating polypeptide, epithelial neutrophil activating peptide, erythropoietin, bone morphogenetic protein, brain-derived neurotrophic factor, BRAK, transforming growth factor beta, and tumor necrosis factor. 
     
     
         5 . The cell tissue gel of  claim 3 , wherein the nutrient is an amino acid, vitamin, mineral, carbon source, fatty acid, or a mixture thereof. 
     
     
         6 . The cell tissue gel of  claim 1 , further comprising one or more matrix factors selected from the group consisting of gelatin, fibronectin, elastin, tenacin, laminin, vitronectin, heparan sulfate, chondroitin, chondroitin sulfate, keratan, keratan sulfate, dermatan sulfate, carrageenan, heparin, chitin, chitosan, alginate, agarose, agar, cellulose, methyl cellulose, carboxyl methyl cellulose, and glycogen. 
     
     
         7 . The cell tissue gel of  claim 6 , further comprising a nutrient or a bioactive agent. 
     
     
         8 . The cell tissue gel of  claim 1 , further comprising a pharmaceutically acceptable excipient. 
     
     
         9 . The cell tissue gel of  claim 8 , wherein the pharmaceutically acceptable excipient is lecithin, petroleum jelly, glycerol, glycerine, or glycerin. 
     
     
         10 . The cell tissue gel of  claim 1 , further comprising an antibiotics or anti-microbial peptide. 
     
     
         11 . The cell tissue gel of  claim 1 , wherein the cell tissue gel is produced by mixing all components of the gel to form a mixture, adjusting the pH of the mixture to weak-acidic to neutral pH, and mixing the mixture until a homogeneous gel is formed. 
     
     
         12 . A method of treating a wound in a subject in need thereof, comprising applying to the wound the cell tissue gel of  claim 1 . 
     
     
         13 . The method of  claim 12 , wherein the concentration of collagen is 1 to 100 mg/ml and the concentration of hyaluronan is 0.5 to 100 mg/mL. 
     
     
         14 . The method of  claim 12 , wherein the cell tissue gel further includes a nutrient or a bioactive agent. 
     
     
         15 . The method of  claim 14 , wherein the bioactive agent is a growth factor selected from the group consisting of epidermal growth factor, fibroblast growth factor, vascular endothelial growth factor, connective tissue growth factor, platelet-derived growth factor, insulin-like growth factor, nerve growth factor, hepatocyte growth factor, colony-stimulating factor, stem cell factor, keratinocyte growth factor, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, glial derived neurotrophic factor, ciliary neurotrophic factor, endothelial-monocyte activating polypeptide, epithelial neutrophil activating peptide, erythropoietin, bone morphogenetic protein, brain-derived neurotrophic factor, BRAK, transforming growth factor beta, and tumor necrosis factor. 
     
     
         16 . The method of  claim 14 , wherein the nutrient is an amino acid, vitamin, mineral, carbon source, fatty acid, or a mixture thereof. 
     
     
         17 . The method of  claim 12 , wherein the tissue gel further includes a pharmaceutically acceptable excipient. 
     
     
         18 . The method of  claim 17 , wherein the excipient is lecithin, petroleum jelly, glycerol, glycerine, or glycerin. 
     
     
         19 . The method of  claim 12 , wherein the tissue gel further includes an antibiotics or anti-microbial peptide. 
     
     
         20 . The method of  claim 12 , wherein the subject is a diabetic patient.