Optimized Method For Producing A Composition Containing Isomaltulose

Abstract

The present invention relates to a method for producing a composition containing isomaltulose from a substrate containing sucrose comprising the steps of: a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass and b) obtaining a composition containing isomaltulose, characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 550 μm. The carrier can be an alginate or a polyvinyl alcohol carrier.

1 . A method for producing a composition containing isomaltulose from a substrate containing sucrose, comprising the steps of:
 a) contacting the substrate containing sucrose with a particulate carrier-immobilized sucrose isomerase biomass, wherein the carrier is an alginate or polyvinyl alcohol carrier and   b) obtaining a composition containing isomaltulose,   characterized in that the median particle size d(0.5) of the carrier-immobilized sucrose isomerase biomass is from 370 to 500 μm.   
     
     
         2 . The method according to  claim 1 , wherein the particulate carrier-immobilized sucrose isomerase biomass has d(0.5) from 420 to 470 μm. 
     
     
         3 . The method according to  claim 1 , wherein the particulate carrier-immobilized sucrose isomerase biomass is spherical. 
     
     
         4 . The method according to  claim 1 , wherein the weight ratio of sucrose isomerase biomass to carrier is 10 to 6 parts sucrose isomerase biomass to 6 to 2 parts of the carrier (in each case dry weight). 
     
     
         5 . The method according to  claim 1 , wherein the composition containing isomaltulose contains trehalulose. 
     
     
         6 . The method according to  claim 1 , wherein the sucrose isomerase biomass is carrier-immobilized by bonding, crosslinking or entrapment immobilization. 
     
     
         7 . The method according to  claim 1 , wherein the sucrose isomerase biomass is a sucrose isomerase, a microorganism cell with sucrose isomerase activity or a cell-extract with sucrose isomerase activity. 
     
     
         8 . The method according to  claim 1 , wherein the sucrose isomerase biomass originates from microorganisms of the genera  Escherichia, Salmonella, Serratia, Erwinia, Enterobacter, Klebsiella, Raoultella, Pectobacterium, Pseudomonas, Azotobacter, Pantoea, Leucanea, Protaminobacter, Leuconostoc, Agrobacterium  or  Bacillus  sp. 
     
     
         9 . The method according to  claim 8 , wherein the microorganism is selected from the group consisting of: Protaminobacter  rubrum, Klebsiella  strain LX3 or strain NK33-98-8,  Klebsiella pneumoniae  strain 342;  Enterobacter  sp. strain SZ62 or strain FMB1,  Erwinia tasmaniensis  strain Et1/99;  Pectobacterium atrosepticum  strain SCRI 1043;  Pectobacterium  carovotum subspecies  brasiliensis  strain PBR 1692,  Azotobacter vinelandii, Leucanea leucocephalia, Erwinia rhapontici, Raoultella planticola, Pseudomonas mesoacidophila, Leuconostoc mesenteroides, Pantoea dispersa, Serratia plymuthica, Serratia marcescens  or  Agrobacterium radiobacter.    
     
     
         10 . Method according to  claim 1 , wherein the method steps a) and b) are carried out in a fixed bed or stirred tank. 
     
     
         11 . The method according to  claim 1 , wherein the obtained composition containing isomaltulose is catalytically hydrogenated. 
     
     
         12 . The method of  claim 1  further comprising a catalytic hydrogenation of the resultant composition containing isomaltulose to obtain a composition containing sugar alcohol. 
     
     
         13 . The method of  claim 13 , wherein the composition containing sugar alcohol is isomalt or an isomalt variant.