Cleaning compositions comprising amylase variants

Abstract

The present invention relates to cleaning compositions including variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor including such compositions, especially at low temperatures.

What is claimed is: 
     
         1 . A cleaning composition comprising:
 a) a variant of a parent alpha-amylase, wherein the variant
 (i) has at least about 80%, but less than 100% sequence identity with the amino acid sequence set forth in any of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, or 8, 
 (ii) comprises a modification at one or more positions corresponding to 109, 1, 7, 280, 284, 320, 323 and 391 of the amino acid sequence set forth in SEQ ID NO: 1, and 
 (iii) has alpha-amylase activity; and 
   b) from about 0.01 to about 99.9 wt % of a cleaning adjunct.   
     
     
         2 . A cleaning composition according to  claim 1  wherein said variant of a parent alpha-amylase comprises a modification at one or more positions corresponding to positions 140, 181, 182, 183, 184, 195, 206, 243, 260, 304, and 476 of the amino acid sequence as set forth in SEQ ID NO: 1. 
     
     
         3 . A cleaning composition according to  claim 1  wherein said variant of a parent alpha-amylase has at least about 80% but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NO: 1. 
     
     
         4 . A composition according to  claim 1  wherein said variant comprises a modification in at least two positions corresponding to positions 109, 1, 7, 280, 284, 320, 323 and 391 of the amino acid sequence as set forth in SEQ ID NO: 1. 
     
     
         5 . A composition according to  claim 1 , wherein said variant comprises a modification in at least two positions selected from the group consisting of 1, 109 and 391. 
     
     
         6 . A composition according to  claim 1  wherein in the variant said modification(s) is/are selected from the group consisting of: X1*, X1A, X7A, X7K, X7E, X7N. X7Q, X7L, X7D, X109A, X109S, X140Y, X181*, X182*, X183*, X184*, X195F, X206Y, X243F, X260G, X280S, X284H, X284R, X284F, X304R, X320A, X320M, X320T, X320V, X320S, X323N, X323R, X323S, X323K, X391A, X391V, and X476K. 
     
     
         7 . A composition according to  claim 1  wherein said variant has an improved wash performance. 
     
     
         8 . A composition according to  claim 1  wherein said variant has at least about 90%, but less than 100%, sequence identity to the amino acid sequence of the parent alpha-amylase. 
     
     
         9 . A composition according to  claim 1  wherein in the variant the number of modifications is 1 to 30. 
     
     
         10 . A composition according to  claim 1  wherein the variant comprises modifications in the positions selected from the group of positions consisting of: X1+X7; X1+X109; X1+X280; X1+X284; X1+X320; X1+X323; X1+X391; X109+X280; X109+X284; X109+X320; X109+X323; X109+X391; X7+X109; X7+X280; X7+X284; X7+X320; X7+X323; X7+X391; X280+X284; X280+X320; X280+X323; X280+X391; X284+X320; X284+X323; X284+X391; X320+X323; X320+X391; and X323+X391, wherein numbering is according to SEQ ID NO: 1. 
     
     
         11 . A composition according to  claim 1  wherein the variant comprises modifications in the positions corresponding to the positions of the amino acid sequence set forth in SEQ ID NO: 2, selected from the group consisting of:
 H1*+G109A+N280S+E391A; 
 H1*+G7K+G109A+N280S+E391A; 
 H1*+G7E+G109A+N280S+E391A; 
 H1*+G7N+G109A+N280S+E391A; 
 H1*+G7Q+G109A+N280S+E391A; 
 H1*+G7L+G109A+N280S+E391A; 
 H1*+G7D+G109A+N280S+E391A; 
 H1*+G109A+N280S+K320A+E391A; 
 H1*+G109A+N280S+K320M+E391A; 
 H1*+G109A+N280S+K320T+E391A; 
 H1*+G109A+N280S+K320V+E391A; 
 H1*+G109A+N280S+M323R+E391A; 
 H1*+G109A+N280S+K320S+E391A; 
 H1*+G109A+N280S+E391V; 
 H1*+G109A+W284R+E391A; 
 H1*+G109A+W284F+E391A; 
 H1*+G109A+N280S+K320A+M323S+E391A; 
 H1*+G109A+N280S+W284F+E391A; 
 H1*+G109A+N280S+M323N+E391A; 
 H1*+G109A+N280S+M323K+E391A; 
 H1*+G109S+N280S+E391A; 
 H1*+G109A+W284H+E391A; 
 H1*+G109A+N280S+K320A+M323N+E391A; 
 H1*+G7A+G109A+N280S+E391A; 
 H1*+G7A+G109A+N280S+W284H+K320A+M323N+E391A; 
 G7A+W284H+K320A+M323N; 
 G7A+K320A+M323N; 
 K320A; 
 G7A+K320A; 
 H1*+G7A+G109A+N280S+E391A; 
 H1*+G109A+N280S+W284H+E391A; 
 H1*+G109A+N280S+M323S+E391A; 
 H1*+G7A+G109A+N280S+K320A+E391A; 
 H1*+G7A+G109A+N280S+M323S+E391A; 
 H1*+G7A+G109A+N280S+M323N+E391A; 
 H1*+G7A+G109A+N280S+W284F+E391A; 
 H1*+G7A+G109A+N280S+W284R+E391A; 
 H1*+G7A+G109A+N280S+K320A+M323S+E391A; 
 H1*+G7A+G109A+W284R+E391A; and 
 H1*+G7A+G109A+N280S+K320A+M323N+E391A. 
 
     
     
         12 . A composition according to  claim 1  wherein the parent alpha-amylase for the variant is selected from the group consisting of alpha-amylases having at least about 90%, identity to any of the amino acid sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, and 8. 
     
     
         13 . A composition according to  claim 12  wherein the parent alpha-amylase for the variant is selected from the group consisting of alpha-amylases having at least about 90% identity to any of the amino acid sequences set forth in SEQ ID NOs: 1 or 2. 
     
     
         14 . A composition according to  claim 1 , wherein the composition is a liquid laundry detergent composition or unit dose detergent composition.